新月弯孢霉对重楼皂苷发酵条件的优化  

Statistical Optimization of Medium Components for Steroid Saponins Biotransformation by Curvularia lunata 3.4381

作　　者：王建玲[1] 刘翊[1] 邹菁[1] 樊欣迎[1] 朱婧[1] 杜连祥[1] 

机构地区：[1]天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《生物技术》2010年第5期69-72,共4页Biotechnology

基　　金：国家自然科学基金项目("基于化学和代谢组学的重楼属药用植物的系统生物学研究";30873378)资助

摘　　要：目的:利用新月弯孢霉对重楼皂苷的转化条件进行优化。方法:采用新月弯孢霉(Curvularia lunata)ATCC3.4381,利用单因素实验对其发酵条件进行优化。采用Plackett-Burman(P-B)方法筛选出对转化率有重要影响的4个因素(蔗糖、酵母膏、(NH4)2SO4和玉米浆的添加量),并采用响应面试验设计(RSM)对重要因素进行优化。结果:最适的培养基条件为:即蔗糖浓度为7.847g/L;酵母膏的浓度为1.969g/L;硫酸铵的浓度为4.824g/L;玉米浆的浓度为11.81g/L。结论:优化后其转化能力得到了明显的提高,转化率为76.80%,并且与拟合值77.18%接近。Objective：A fermentation medium for steroid saponins biotransformation by Curvularia lunata 3.4381 had been optimized.Method：Important components and their concentrations were investigated using fractional factorial design and Plackett-Burman（P-B）.The results showed that sucrose,corn pulp,yeast extract and （NH4）2SO4 are important for biotransformation.A polynomial model related to medium components and steroid saponins yield had been established.Result：The significance of tested components was related to sucrose （7.847g/L）,yeast extract （1.969g/L）,（NH4）2SO4 （4.824g/L） and corn pulp （11.81g/L）.Conclusion：The percent of conversion reached 76.80% （p0.05）,which is close to the fitting value 77.18%.

关 键 词：Plackett-Burman设计法 响应面法 新月弯孢霉 

分 类 号：TQ467.95[化学工程—制药化工]