Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding  

蹄蝠科的核型进化:比较染色体涂色、G带和C带分析(英文)

作　　者：毛秀光[1,2] 王金焕[1] 苏伟婷[1] 王应祥[1] 杨凤堂 佴文惠[1] 

机构地区：[1]中国科学院昆明动物研究所遗传资源与进化国家重点实验室,云南昆明650223 [2]中国科学院研究生院,北京100049 [3]Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus

出　　处：《Zoological Research》2010年第5期453-460,共8页动物学研究（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(30770293);the Ministry of Science and Technology of China(2005DKA21502)

摘　　要：Comparing to its sister-family （Rhinolophidae）, Hipposideridae was less studied by cytogenetic approaches. Only a few high-resolution G-banded karyotypes have been reported so far, and most of the conclusions on the karyotypic evolution in Hipposideridae were based on conventional Giemsa-staining. In this study, we applied comparative chromosome painting, a method of choice for genome-wide comparison at the molecular level, and G- and C-banding to establish comparative map between five hipposiderid species from China, using a whole set of chromosome-specific painting probes from one of them （Aselliscus stoliczkanus）. G-band and C-band comparisons between homologous segments defined by chromosome painting revealed that Robertsonian translocations, paracentric inversions and heterochromatin addition could be the main mechanism of chromosome evolution in Hipposideridae. Comparative analysis of the conserved chromosomal segments among five hipposiderid species and outgroup species suggests that bi-armed chromosomes should be included into the ancestral karyotype of Hipposideridae, which was previously believed to be exclusively composed of acrocentric chromosomes.与其姐妹科(菊头蝠科)相比,蹄蝠科的细胞遗传学研究较少。迄今为止,仅少数蹄蝠科几个物种有高分辨率的G带核型报道,且有关该科核型进化的大多数结论都是基于常规Giemsa染色研究而得。该研究利用三叶小蹄蝠的染色体特异探针,通过比较染色体涂色、G和C显带,建立了5种蹄蝠的染色体同源性图谱,并探讨了它们同源染色体间的G和C带异同。结果表明:罗伯逊易位、臂内倒位以及异染色质的扩增可能是蹄蝠科物种核型进化的主要机制。通过对这5种蹄蝠物种及其外群物种之间的同源染色体片段的比较分析,作者推测蹄蝠科的祖先核型并不像先前认为的全由端着丝粒染色体组成,而应该含有中着丝粒染色体。

关 键 词：Comparative chromosome painting Robertsonian translocation Paracentric inversion Hipposideridae CHIROPTERA 

分 类 号：Q343.2[生物学—遗传学] Q959.833