水稻多分蘖突变体ht1的遗传分析和分子定位  

Genetic analysis and molecular mapping of a high-tillering mutant (ht1) in rice

作　　者：李万昌[1] 王永飞[2] 马三梅[2] 郭士伟[3] 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]暨南大学生物工程学系,广州510632 [3]江苏省农业科学院粮食作物研究所江苏省优质水稻工程技术研究中心,南京210014

出　　处：《遗传》2010年第10期1065-1070,共6页Hereditas（Beijing)

基　　金：河南省科技攻关项目(编号:102102310318);国家重大转基因专项(编号:2009ZX08009-104B)资助

摘　　要：在水稻品种新稻18中发现了一个多分蘖植株,经过多代自交获得了稳定的多分蘖突变株,突变体ht1在整个生育期最显著的特点就是分蘖数目多,是其野生型新稻18的3倍以上。遗传分析表明该基因受1对显性核基因控制,命名为HT1。利用微卫星标记将HT1初步定位于第10号染色体RM25435和RM25552之间,进一步利用极端个体定位法把HT1精细定位于标记RM25523和RM25532之间,HT1基因距它们的遗传距离均为0.05cM,这两标记间的物理距离约为130kb。A rice（Oryza sativa L.） mutant with a high-tillering capacity,designated as ht1,was found from a japonica rice variety Xindao18.This high-tillering mutant phenotype was stably expressed through successive five self-crossed generations,and the number of tillers in mutants was three times more than that in wild-type rice.Genetic analysis showed that the phenotype of ht1was controlled by a single dominant nuclear gene,temporarily designated as HT1.By means of molecular marker technique,the HT1 gene was mapped to an interval between two SSR markers RM25435 and RM25552 on chromosome 10.Through high-resolution linkage analysis,the HT1 gene was further restricted to a 0.1 cM region flanked by two SSR markers RM25523 and RM25532.The physical distance between these two markers is about 130kb.

关 键 词：水稻 多分蘖 遗传分析 分子定位 

分 类 号：S511[农业科学—作物学]