肉毒毒素A腺体内注射对大鼠前列腺增生组织的影响  

Effects of intraprostatic injection of botulinum toxin A (BTX-A) on benign prostate hyperplasia

作　　者：徐耀鹏[1] 余虓[2] 叶章群[2] 潘铁军[1] 文瀚东[1] 王涛[1] 

机构地区：[1]广州军区武汉总医院泌尿外科,湖北武汉430070 [2]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430031

出　　处：《中华男科学杂志》2010年第10期905-910,共6页National Journal of Andrology

摘　　要：目的:探讨肉毒毒素A(BTX-A)腺体内注射对大鼠前列腺增生模型的影响。方法:SPF级雄性SD大鼠64只,丙酸睾酮造模成功后,随机均分为BTX-A高、中、低(20U、10U、5U,n=12)剂量组和阴性对照组(生理盐水,n=12)行腺体内注射,假手术组(n=12)为对照。注射后2周和4周切取前列腺,观察各组腺体外观、腹侧叶称重。HE染色观察组织学改变;千屏病理图像分析系统半定量检测各组腺体面积、间质面积。结果:BTX-A20U注射后3d内有2只大鼠死亡,余均存活。2周后,与阴性对照组相比,高、中、低剂量组大鼠前列腺明显萎缩(P<0.01,0.01,0.05),重量减轻,光镜下腺管上皮细胞萎缩,腺管面积和间质面积均缩小。4周后影响有所降低,以5UBTX-A组大鼠最为明显。结论:大鼠前列腺内注射BTX-A可使前列腺体积缩小,组织学变化明显,但大剂量有可能导致大鼠死亡。Objective：To investigate the effects of intraprostatic injection of botulinum toxin A （BTX-A） on benign prostate hyperplasia （BPH） in rats. Methods：Models of BPH were established in adult male Sprague-Dawley rats by injection of testosterone propionate,and then divided into three BTX-A groups,injected with BTX-A into the ventral prostate at the doses of 5 U,10 U and 20 U,a negative control group,injected with saline only,and a sham operation group,with 12 in each. The prostates of the animals were harvested at 2 or 4 weeks after the injection,their volumes and weights measured,histological changes examined by HE staining,and glandular and interstitial areas semi-quantified by the image analysis system. Results：Two rats died in the 20 U group within 3 days after BTX-A injection. Compared with the saline group,the 5 U,10 U and 20 U BTX-A groups showed significant decreases in prostatic volume （P 0.01,0.01 and 0.05）,weight,and glandular and interstitial areas as well as atrophic epithelia in the glandular tube at 2 weeks. These changes were lessened at 4 weeks,especially in the 5 U group. Conclusion：Intraprostatic injection of BTX-A induces obvious atrophy and histological changes of the prostate,but meanwhile may potentially result in death at a large dose.

关 键 词：肉毒毒素A 前列腺增生 组织学 大鼠 

分 类 号：R697.32[医药卫生—泌尿科学] R-332[医药卫生—外科学]