维生素E抑制3T3-L1前脂肪细胞的分化  

Inhibitory effect of vitamin E on differentiation of 3T3-L1 preadipocyte

作　　者：郑奕迎[1] 刘声远[2] 马兰[1] 龙儒桃[1] 

机构地区：[1]海南医学院病理生理学教研室,海南海口571101 [2]华中科技大学同济医学院病理生理学系,湖北武汉430030

出　　处：《海南医学院学报》2010年第9期1117-1119,共3页Journal of Hainan Medical University

基　　金：海南省自然科学基金资助项目(808164)~~

摘　　要：目的:研究维生素E对3T3-L1前脂肪细胞增殖和分化的影响。方法:利用MTT研究维生素E对3T3-L1前脂肪细胞增殖的影响,利用油红O染色法测定维生素E对3T3-L1前脂肪细胞分化的影响。结果:维生素E对3T3-L1前脂肪细胞增殖无影响,10～100μmol/L维生素E能抑制3T3-L1前脂肪细胞的分化。结论:维生素E对前脂肪细胞分化具有抑制作用。Objective：To study the effects of vitamin E on proliferation and differentiation of 3T3-L1 preadipocyte.Methods：The effect of vitamin E on the proliferation of 3T3-L1 was determined by MTT.Oil red O staining was used to determine the differentiation rate and level of 3T3-L1 preadipocytes.Results：Vitamin E inhibited the differentiation of preadipocytes at 10 μmol/L and 100 μmol/L.Conclusions：Vitamin E could inhibit the differentiation of 3T3-L1 preadipocyte.

关 键 词：维生素E 碘 3T3-L1 增殖 分化 肥胖 MTT 油红O染色法 

分 类 号：Q581[生物学—生物化学] Q254