左旋四氢巴马汀对脑缺血-再灌注损伤大鼠的保护作用  

Protective Effect of L-tetrahydropalmatine on Focal Cerebral Ischemia Reperfusion Injury in Rats

作　　者：陈鹏[1] 弥曼[1] 李斌[2] 

机构地区：[1]西安医学院,710021 [2]西安医学院附属医院胸外肿瘤科,710077

出　　处：《医药导报》2010年第11期1405-1408,共4页Herald of Medicine

基　　金：陕西省卫生厅2006年度自然科学项目(基金编号:06018);西安医学院2006年度院扶植项目(项目编号:2006FZ1)

摘　　要：目的探讨左旋四氢巴马汀(L-THP)对脑缺血-再灌注损伤大鼠的保护作用及其机制。方法将60只SD大鼠随机分为假手术组(sham组)、模型组(I-R组)、尼莫地平组(I-R+Nim10mg·kg)、L-THPI组(I-R+L-THP50.0mg·kg-1)、L-THPⅡ组(I-R+L-THP25.0mg·kg-1)、L-THPⅢ组(I-R+L-THP12.5mg·kg-1),每组10只。除sham组外,其他组采用大脑中动脉阻塞法建立大鼠局灶性脑缺血-再灌注模型。对大鼠进行行为缺陷评分,测定脑梗死范围、脑水肿体积、血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果局灶性脑缺血-再灌注损伤致大鼠神经行为学评分、脑梗死体积、脑水肿体积增加,血清SOD活性降低,MDA含量增高。L-THP可明显改善上述指标,其中L-THPI、Ⅱ组与模型组比较均差异有极显著性(均P<0.01),L-THPⅢ组与模型组比较,差异有显著性(P<0.05)。结论 L-THP可减轻大鼠脑局灶性脑缺血-再灌注损伤,其机制与减轻自由基对脑组织的损害有关。Objective To study the protective effects of different doses of L-tetrahydropalmatine（L-THP） on focal cerebral ischemia reperfusion injury in rats and its mechanism.Methods Sixty healthy SD rats were randomly divided into six groups：①sham operation,②ischemia reperfusion model（I-R）,③nimodipine group（I-R＋Nim10 mg·kg^-1）,④large dose of L-tetrahydropalmatine group （I-R＋L-THP50 mg·kg^-1）,⑤ middle dose （I-R＋L-THP25 mg·kg^-1）,⑥ low dose （I-R＋L-THP 12.5 mg·kg^-1）.We used the Modifiied Longa method to make the model of focal cerebral ischemia reperfusion injury in rats,except the sham operation ones.Infarct size,hydropic volume of brain,neurological deficit scores,superoxide dismutase（SOD） activity,malondialdehyde（MDA） content in serum of rats were measured.Results The results indicated that the ischemia reperfusion injury resulted in high neurological deficit scores,large brain infact size and hydropic volume,decreased activity of SOD and increased content of MDA.It was showed that different doses of L-THP improved all signs mentioned above.Compared with the model control,L-THP I and II groups had significantly statistical meaning （P0.01）,L-THP III was P0.05.Conclusion L-THP has the protective effects on rat brain damage induced by focal cerebral ischemia reperfusion,the mechanism of which might be associated with attenuating the injury by free radicals.

关 键 词：四氢巴马汀 左旋 缺血-再灌注损伤 脑 梗死 水肿 自由基 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]