荧光定量聚合酶链反应定量检测乙型肝炎病毒-DNA与酶联免疫吸附试验定性检测乙型肝炎病毒血清标志物的对比分析  

The relationship between quantitative detection of hepatitis B virus DNA with fluorescent quantitative polymerase chain reaction and qualitative detection of hepatitis B virus markers with enzyme-linked immunosorbent assay

作　　者：胡淑芬 梁嘉琪 高慧 王莉 

机构地区：[1]广东省广州市番禺区中心医院,511400

出　　处：《实用医技杂志》2010年第11期1012-1014,共3页Journal of Practical Medical Techniques

摘　　要：目的探讨血清中乙型肝炎病毒(HBV)-DNA与乙型肝炎病毒血清标志物(HBV-M)之间的关系及临床意义。方法采用荧光定量聚合酶链反应(FQ-PCR)和酶联免疫吸附测定试验(ELISA)法分别检测845例患者的血清HBV-DNA和HBV-M含量,并对其结果进行统计对比分析。结果 845份患者血清标本中,乙型肝炎病毒表面抗原阳性(HBsAg+)、乙型肝炎病毒e抗原阳性(HBeAg+)、乙型肝炎病毒核心抗体阳性(HBcAb+)模式(1.3.5.模式)的HBV-DNA阳性率为87.5%,HBV-DNA含量以中、高拷贝为主;HBsAg+、乙型肝炎病毒e抗体阳性(HBeAb+)、HBcAb+模式(1.4.5.模式)的HBV-DNA阳性率为26.3%,HBsAg+、HBcAb+模式(1.5.模式)的HBV-DNA阳性率为32.4%,其含量均以中、低拷贝数为主。1.3.5.模式患者血清HBV-DNA含量和阳性率与1.4.5.模式和1.5.模式差异均有统计学意义(P<0.05);1.4.5.模式与1.5.模式患者血清之间的HBV-DNA含量和阳性率差异无统计学意义(P>0.05)。结论 HBeAg和HBV-DNA联合检测,更能反映乙型肝炎患者HBV感染、传染性及体内复制情况。Objective To analyze the relationship between the hepatitis B virus DNA（HBV-DNA）and hepatitis B virus markers（HBV-M）.Methods HBV-DNA and HBV-M in 845 cases of serum were quantitatively detected with fluorescent quantitative polymerase chain reaction（FQ-PCR） and qualitatively detected with enzyme-linked immunosorbent assay（ELISA）.Results In the 845 cases,the HBV-DNA positive rate in hepatitis B surface antigen（HBsAg＋）,hepatitis B e antigen（HBeAg＋）,hepatitis B core antibody（HBcAb＋）group（mode 1.3.5.）was 87.5%（mainly made up by middle and high copy）,in the HBsAg＋,hepatitis B e antibody（HBeAb＋）,HBcAb＋ group（mode 1.4.5.）was 26.3% and in the HBsAg＋,HBcAb＋ group（mode 1.5.）was 32.4%（mainly made up by low and middle copies in last two groups）.The content and positive rate of HBV-DNA in mode 1.3.5.group was different from that in mode 1.4.5.group and mode 1.5.group statistically（P〈0.05）,and no difference between that in mode 1.4.5.group and mode 1.5.group（P〉0.05）.Conclusion It is more significant to jointly detect HBeAg and HBV-DNA to reflect the real conditions of HBV infection and replication.

关 键 词：肝炎 乙型 DNA 聚合酶链式反应 酶联免疫吸附测定 

分 类 号：R512.62[医药卫生—内科学]