紫花苜蓿种子人工加速老化方法研究  

Study on Optimizing the Accelerated Ageing Condition of Alfalfa Seeds

作　　者：毛培胜[1] 李寅菲[1] 

机构地区：[1]中国农业大学动科学院草业科学系,北京100193

出　　处：《种子》2010年第10期10-14,共5页Seed

基　　金：现代牧草产业体系(nycytx-037);行业性公益项目"人工草地优质牧草生产技术研究与示范"(nyzx07-022);"十一五"科技支撑课题"中部温带地区优质高产抗逆牧草新品种选育及产业化示范(2008BADB3B05)"支持

摘　　要：以不同发芽水平的3份紫花苜蓿种子为材料,研究比较老化温度(39、41、43、45℃)和时间(24、36、48、60、72、84、96h)处理对种子发芽率的影响,筛选确定紫花苜蓿种子进行人工加速老化测定的适宜条件,并建立标准化的测定程序。结果表明:随着老化温度的升高,紫花苜蓿种子发芽率呈现逐渐下降的趋势,45℃老化处理可以显著降低种子老化后的发芽率(p<0.05)。随着老化时间的延长,Lot1、Lot2和Lot3紫花苜蓿种子发芽率逐渐下降,且在老化36～84h间发芽率差异不显著(p>0.05)。虽然老化温度的提高可以促进种子的老化,但受老化前种子发芽率的影响,其中发芽率高的种子样品Lot1在老化84h内变化较小,而发芽率低的Lot3在老化36h后就开始出现明显变化。老化温度与时间的互作效应对紫花苜蓿种子老化发芽率的影响极显著(p<0.01),且老化温度和老化时间均促进种子老化。紫花苜蓿种子加速老化处理的适宜条件是45℃老化84h。Three samples of alfalfa（Medicago sative L）seeds with different germination level were used in the experiment and conducted to analyse the effect of the aging temperature（39,41,43,45℃）and aging duration（24,36,48,60,72,84,96h）on the germination percentage,and to find out the suitable condition and procedure for the accelerated aging test of alfalfa seed.The results showed that the germination rate decreased gradually with the ageing temperature increasing,and germination rate after ageing was decreased significantly（p〈0.05）at 45℃.The seed germination of Lot1,Lot2 and Lot3 gradually decreased with the ageing duration prolonging,but there were not significant differen（p〈0.05）from ageing duration 36h to 84h.Although ageing temperature could promote the seed ageing,the germination rate after ageing of Lot1 seeds with higher germination percentage changed slowly within ageing duration 84h,and that of Lot3 with lower germination percentage changed quickly beyond ageing 36h.The interaction between the ageing temperature and duration was significantly related（p〈0.01）with the germination rate after ageing of alfalfa seed,ageing temperature and duration were all the key factor for promoting the seed ageing.The result indicated that ageing temperature 45℃ and ageing duration 84h was suitable condition for accelerated ageing test of alfalfa seed.

关 键 词：加速老化 种子活力 紫花苜蓿 

分 类 号：S551.7[农业科学—作物学]