薰衣草经嗅觉通路吸入对小鼠抑郁样行为改变及其作用机制初步探讨  

Influence of Lavender Inhalation by the Olfactory Pathway on Depression-Like Behavioral Changes in Mice

作　　者：牛利华[1] 胡庆东[1] 徐金勇[1] 刘利茵[1] 李光武[1] 顾怀宇[2] 

机构地区：[1]安徽医科大学神经生物研究所,安徽合肥230032 [2]中山大学医学院人体解剖与神经生物学教研室,广东广州510000

出　　处：《环境与健康杂志》2010年第10期861-865,F0003,共6页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(81000589);安徽省自然科学基金资助项目(090413145);国家同步辐射实验室"教育部创新计划"同步辐射研究生创新基金资助项目

摘　　要：目的研究薰衣草对抑郁症小鼠行为学及脑内单胺类神经递质的影响,探讨薰衣草通过嗅觉通路对抑郁症的干预作用及其可能的机制。方法采用慢性不可预见性中等强度应激(CUMS)+孤养的方法建立抑郁症模型,建模成功后小鼠随机分为3组,分别为抑郁对照组、抑郁+纯净水吸入组和抑郁+薰衣草吸入组,每组8只,另设一空白对照组,空白对照组小鼠可正常摄食、饮水,不给予任何刺激。抑郁+薰衣草精油吸入组持续吸入雾化薰衣草水溶液1h,每天3次;抑郁对照组不给任何药物;抑郁+纯净水吸入组吸入雾化的纯净水1h,每天3次。连续暴露4周,并分别在造模前、后及暴露的1、2、3、4周记录体重,进行悬尾实验、强迫游泳实验和糖水消耗实验。HE染色观察脑区神经元的形态变化,c-fos、c-jun、5-羟色胺(5-HT)免疫组化观察脑内单胺类神经递质的变化。结果与造模后相比,抑郁+薰衣草吸入组在暴露3周后悬尾实验不动时间明显缩短(P<0.01),且接近空白对照组(P>0.05);暴露1周后强迫游泳实验不动时间明显缩短(P<0.01),在暴露3周后接近空白对照组(P>0.05);暴露2周后糖水消耗量有明显增加(P<0.01),且接近空白对照组(P>0.05)。与空白对照组比较,抑郁对照组、抑郁+薰衣草精油吸入组海马、下丘脑、杏仁核HE染色可见明显的胶质细胞数量减少,细胞核蓝染较淡。免疫组化结果显示,与抑郁对照组比较,抑郁+薰衣草精油组小鼠的下丘脑、杏仁核、海马内5-HT、c-fos、c-jun表达均有明显增加。结论薰衣草精油吸入可以缓解小鼠的抑郁样行为。Objective To know the influence of lavender on the depression behavior of mice and monoamine neurotransmitters, and explore the remedial effect of lavender on depression through olfactory pathway. Methods Moderate chronic unpredictable stress （CUMS）＋solitary ways was used to support the establishment of depression model. The normal control group： feeding water, without any stimulation, modeling successful mice were divided into 3 groups, 8 in each. Lavender inhalation group： inhaled lavender for 1 hour at one time, three times a day for 4 consecutive weeks; Depression control group：without lavender inhalation; purified water inhalation group：treated with purified water through atomized inhalation; The control group：no any treatment. Tail suspension test and forced swimming test in the fixed time and sugar consumption measurement were conducted. The changes of e- fos,c-jun,5-HT in brain tissue was determined by immunohistochemistry. Results In depression＋lavender inhalation group,after 3 weeks of treatment, the immobility time shortened significantly compared with the model without treatment （P〈0.01）, closed to the control group （P〉0.05）; after one week of treatment, forced swimming test time was significantly shortened （P〈0.01）, after 3 weeks, it closed to the control group（P〉0.05）; after 2 weeks of treatment, the sugar consumption increased significantly（P〈0.01）. Compared with the control group, obvious decrease in the number of glial cells nuclei in hippocampus, hypothalamus was seen in depression and depression ＋lavender groups, and 5-HT,c-fos,c-jun expression in hypothalamus,amygdala,hippocampus were significantly increased in lavender inhalation group compared with depression group. Conclusion Inhalation lavender essential oil may relief the depression-like behavior in mice.

关 键 词：薰衣草 抑郁症 嗅觉通路 芳香疗法 

分 类 号：R994.6[医药卫生—毒理学]