长波紫外线暴露致大鼠神经细胞能量代谢降低的恢复实验研究  

Recovery Experiment of Ultraviolet A-Induced Energy Metabolism Decrease in Cells in Culture

作　　者：吴瑞[1] 白雪涛[2] 

机构地区：[1]首都医科大学生物医学工程学院物理教研室,北京100069 [2]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《环境与健康杂志》2010年第10期878-879,共2页Journal of Environment and Health

摘　　要：目的利用体外培养神经元探讨长波紫外线(UVA)对细胞能量代谢的影响以及去除UVA暴露后,细胞能量代谢的恢复。方法将体外原代培养的大鼠神经元暴露于UVA,剂量分别为0、3、9J/cm2。照射后继续培养4和28h,以细胞化学方法进行单细胞细胞色素氧化酶活力测定,用图像分析仪进行定量分析。结果各暴露组细胞经过UVA照射及4h恢复培养后,与对照组相比,光镜下,生命征象未见明显改变,细胞色素氧化酶活力随照射剂量增加明显下降。UVA照射后,经28h恢复培养,3J/cm2剂量组细胞色素氧化酶活力与对照组未见显著差别。9J/cm2剂量组虽有所恢复,但未达到对照组水平。结论低剂量UVA暴露在未导致细胞出现明显损伤情况下,可使细胞能量代谢明显降低。经去除UVA暴露,在一定时间内,较低剂量暴露致细胞能量代谢降低可明显可恢复;但较高剂量暴露后,短期内难以恢复至正常水平。Objective To know the effect of ultraviolet A on energy metabolism in the ceils in culture by using the primary cultured postnatal rat cortex neurons for in which there were many mitoehondfia and whether UVA-indueed cell energy metabolism decrease would recover when the UVA exposure was eliminated. Methods The primary cultured postnatal SD rat cortex neurons in 60mm dishes were exposed to UVA in a chamber at the doses of 0, 3 and 9 J/cm^2 respectively. The exposure was followed by 4 and 28 hours culture and then the activity of cytochrome oxidase （COX） in the single neuron was determined by cytochemistry, the quantitative analysis was done with hnage-Pro plus 4.0. Results In exposure groups, after UVA exposure followed by 4 h culture, the activity of COX in the neurons decreased as the UVA exposure increased and no significant changed vital signs in the cells were found under the microscope. 28 h of culture after UVA exposure, in 3 J/cm^2 group, the decreased activity of COX increased to the level of control group, while in 9 J/cm^2 group, it did not recover significantly. Conclusion Low level UVA exposure can cause obvious energy metabolism decrease in the cells in culture although the significant changed vital signs are not seen. In lower exposure level, the UVA-induced cell energy metabolism decrease can recover within a certain time as the UVA exposure is eliminated, and in higher exposure level, the significant recovery will be difficult.

关 键 词：紫外线 能量代谢 恢复 细胞 

分 类 号：R994.6[医药卫生—毒理学]