模拟微通道光生物反应器内光合细菌的成膜及产氢特性  

Film formation and hydrogen production performance of photosynthetic bacteria in simulating microchannels photo-bioreactor

作　　者：朱恂[1] 高晓玉[1] 屈晓凡[1] 王永忠[1] 田鑫[1] 廖强[1] 

机构地区：[1]重庆大学工程热物理研究所,重庆400030

出　　处：《化工学报》2010年第11期2965-2970,共6页CIESC Journal

基　　金：国家杰出青年基金项目(50825602);国家自然科学基金项目(50806086;20876183)~~

摘　　要：In order to understand the micro-behavior and mass transfer of photosynthetic bacteria(PSB)in an immobilized granule,a visualized microchannels photo-bioreactor was designed to observe the adsorption and film forming of PSB named as Rhodopseudomonas palustris CQK 01.And the effects of substrate concentration and flow rate on hydrogen production and substrate degradation of microchannels photo-bioreactor were investigated.The results showed that the PSB behavior in the microchannels can be divided into four stages:initial diffusion and adsorption,diffusion-adsorption and initial splitting and reproducing of bacteria,dividing-increasing and morphological transformation,and gathering and biofilm forming.With the increase of substrate concentration and flow rate,the rate and yield of hydrogen producing rised first and then fell down,but the substrate conversion monotonically went up with its rate slowed down.The substrate degradation efficiency increased first and then decreased with increase of substrate concentration,and reduced with increase of solution flow rate.At higher substrate concentration and flow rate,substrate inhibition and the loss of biofilm were the main reasons for lower rate and yield of hydrogen production.In the range of present experiments,the optimum substrate concentration and flow rate were 50 mmol·L-1 and 2.1 ml·h-1 respectively,the maximum rate and yield of hydrogen production were 2.4 mmol·(g cell dry mass)-1·h-1 and 0.78 mol H2·(mol sub)-1 respectively.In order to understand the micro-behavior and mass transfer of photosynthetic bacteria（PSB）in an immobilized granule,a visualized microchannels photo-bioreactor was designed to observe the adsorption and film forming of PSB named as Rhodopseudomonas palustris CQK 01.And the effects of substrate concentration and flow rate on hydrogen production and substrate degradation of microchannels photo-bioreactor were investigated.The results showed that the PSB behavior in the microchannels can be divided into four stages：initial diffusion and adsorption,diffusion-adsorption and initial splitting and reproducing of bacteria,dividing-increasing and morphological transformation,and gathering and biofilm forming.With the increase of substrate concentration and flow rate,the rate and yield of hydrogen producing rised first and then fell down,but the substrate conversion monotonically went up with its rate slowed down.The substrate degradation efficiency increased first and then decreased with increase of substrate concentration,and reduced with increase of solution flow rate.At higher substrate concentration and flow rate,substrate inhibition and the loss of biofilm were the main reasons for lower rate and yield of hydrogen production.In the range of present experiments,the optimum substrate concentration and flow rate were 50 mmol·L-1 and 2.1 ml·h-1 respectively,the maximum rate and yield of hydrogen production were 2.4 mmol·（g cell dry mass）-1·h-1 and 0.78 mol H2·（mol sub）-1 respectively.

关 键 词：光合细菌 微通道 成膜 浓度 流速 产氢 降解 

分 类 号：TK91[动力工程及工程热物理]