一株韩国新型DHV的分离及RT-PCR鉴定  

Isolation and RT-PCR identification of a new-type Duck hepatitis virus

作　　者：马秀丽[1] 赵立娜[2] 夏雪梅[3] 吴静[1] 林树乾[1] 姜亦飞[1] 

机构地区：[1]山东省农业科学院家禽研究所,山东省家禽疫病诊断与免疫重点实验室,山东济南250023 [2]山东农业大学动物科技学院,山东泰安271018 [3]凯里学院环境与生命科学学院,贵州凯里556011

出　　处：《浙江农业学报》2010年第5期596-598,共3页Acta Agriculturae Zhejiangensis

基　　金：山东省自然基金项目(Z2006D06);家畜疫病病原生物学国家重点实验室开放课题

摘　　要：从山东省某疑似鸭肝炎发病区分离到1株病毒JFX08,血清中和试验结果表明该分离毒与传统的Ⅰ型鸭肝炎病毒无交叉保护,分离毒回归3日龄雏鸭,可复制出鸭肝炎的典型症状和病理变化。根据已公布的韩国新型（基因C型）DHV的序列设计合成一对引物,进行RT-PCR扩增,得到大小约414 bp的目的条带;测序分析发现,分离毒与传统Ⅰ型DHV之间的相似性较低,而与韩国新型DHV之间的相似性高达93.2%~94.0%;进化分析结果表明,该分离毒与韩国新型DHV的遗传距离最近,属基因C型DHV。One stain of Duck hepatitis virus was isolated from Shandong Province and named as JFX08.The results of serum neutralization test showed that there was no cross-protection occurred in strain JFX08 and Duck hepatitis virus typeⅠ（DHV-Ⅰ）.Clinical symptom and pathological lesion could be reproduced in 3-day ducklings challenged by the isolated strain.A pair of primers was designed and synthesized according to the sequence of DHV and a reverse transcriptase polymerase chain reaction method was developed for strain JFX08.The identity analysis of nucleotide sequence between strain JFX08 and N-DHV from Korean（genotype C） showed the similarity was 93.2%-94.0%.However,the nucleotide sequence identity between strain JFX08 and DHV-Ⅰ was much lower.Phylogenetic and evolutionary analysis showed that the genetic distance between the isolated strain JFX08 and N-DHV from Korean was the nearest,indicating JFX08 belonged to genotype C,which was a different branch from genotype A and B.

关 键 词：新型DHV 分离 RT-PCR 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]