绵羊肺腺瘤病毒内蒙株env基因的克隆及生物信息学分析  

CHONING AND SEQUENCE ANALYSIS OF ENV GENOMIC SEQUENCE FROM THE INNER MONGOLIA STRAIN OF EXDOGENOUS JAAGSIEKTE SHEEP RETROVIRUS

作　　者：韩硕[1] 刘淑英[1] 

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2010年第3期5-9,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(30760178);国家自然科学基金(30960271)

摘　　要：根据GenBank中绵羊肺腺瘤病毒(AF105220)env基因序列设计合成了1对引物。提取自然感染绵羊肺腺瘤病的羊肺组织总RNA,应用RT-PCR技术对env基因进行了扩增,将其回收后克隆入PMD19-T载体中并进行了序列测定。应用生物信息学分析技术并对env基因所编码的蛋白进行了结构预测。结果表明,env基因全长1 848bp,共编码615个氨基酸。在基因编码的TM区发现外源性exJSRV特异性的"YXXM"基序。测定序列与美国代表株(AF105220)的env基因序列比较核苷酸同源性为98.8%,推导出的氨基酸同源性为99.8%。通过protparam工具统计分析可知env基因所编码的蛋白为亲水蛋白。According to the published env gene sequence of exJSRVstrain in GenBank,a Pair of primers were designed.The total RNA was extracted from natural sheep pulmonary adenomatosis cases.A env gene was amplified by RT-PCR and then was cloned into PMD19-T vector and then sequenced.The acquired nucleotide sequences were analysed by the DNASTAR software and on line tool in biological information.The results indicated that,A 1848bp long cDNA sequence was obtained and coded a total of 615 aminoacids.The YXXM motif was found in the region of TM,which are reliable molecular markers for the infectious exogenous virus.The nucleotide and amino acid sequences of NM strain were compared with the USA strain（AF105220）,The nucleotide and amino acid homology were 98.8% and 99.8%.Statistical analysis shows that env gene encoded hydrophilic protein.

关 键 词：绵羊肺腺瘤病毒 ENV基因 克隆 序列分析 

分 类 号：S826[农业科学—畜牧学] Q785[农业科学—畜牧兽医]