纤维素高效降解真菌-细菌复合系的筛选及其分解特征  

CHARACTERIZATION AND SELECTION OF BACTERIA-FUNGI CONSORTIA FOR DEGRADING CELLULOSE EFFECIENTLY

作　　者：何一平[1] 袁博[1] 王瑞刚[1] 冯福应[1] 刘惠荣[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2010年第3期177-180,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金项目"内蒙古荒漠区纤维素降解细菌分子生态学研究"(No.30860015);教育部春晖计划项目"甜高粱秸秆纤维素液态能源生物转化技术"(No.Z2007-1-01017);内蒙古自治区重大科技专项"甜高粱秸秆乙醇生物技术与产业化"国际合作项目"旱寒荒漠区甜高粱秸杆贮藏及其燃料乙醇产业化技术支撑";地方合作项目"五原县甜高粱生物质能源途径的建立与应用"

摘　　要：真菌-真菌或细菌-细菌复合系可提高纤维素的降解,但人们对真菌与细菌组成复合系的降解情况知之甚少。本文以真菌(LF1,LF3)和细菌(DS6,DS9)各2株组合形成11个复合系,通过比较单一菌种及各复合系在常温20℃下的羧甲基纤维素(CMC)酶活和滤纸糖酶(FPA)酶活来分析分解特征,进而确定高效降解复合系。结果表明,CMC酶活和FPA酶活随时间的变化规律基本一致,单一菌种中LF3最大CMC酶活(Mc)和最大FPA酶活(Mf)为最高,分别为1.99 U和1.18 U;除LF3与细菌组成的复合系之外,其余复合系的最大酶活均明显高于其所含菌种单一培养时的,复合系L13(含LF1和LF3)的CMC和FPA酶活均于168 h达到最大,且在所有复合系中其Mc和Mf最高,分别达到了3.95 U和2.23 U;复合系D6L13(含DS6、LF1和LF3)的Mc和Mf仅次于L13,分别达到了3.54 U和1.82 U,出现时间分别为168 h和144 h。People know little about the cellulose degradation by fungi-bacteria consortia,though the efficiency of degrading cellulose can be improved with the application of fungi-fungi or bacteria-bacteria consortia.For characterization and selection consortia of degrading cellulose efficiently,this paper constructed 11 consortia of fungi（strains LF1 and LF3） and bacteria（strains DS6 and DS9） and compared the CMC and FPA enzyme activities of all single-cultured strains and consortia under normal temperature of 20℃.The results showed that the changing trends of CMC enzyme activity was principally in agreement with that of FPA.The maximum CMC enzyme activity（Mc） and FPA enzyme activity（Mf） of LF3,among all single strain,were highest with the values of 1.99 U and 1.18 U respectively.The Mc and Mf were generally higher than ones of their correspondent single strain,but with the exception of consortia including LF3 and bacteria.Consortia D6L13（including strains DS6,LF1 and LF3） showed a little lower Mc（3.54 U,at 168 h） and Mf（1.82 U,at 144 h） than those of LF13（including strains LF1 and LF3） which had the highest Mc（3.95 U,at 168 h） and Mf（2.23 U,at 168 h） among all consortia.

关 键 词：纤维素降解 真菌 细菌 复合系 CMC酶活 FPA酶活 

分 类 号：Q75[生物学—分子生物学]