健儿强身膏对哮喘豚鼠血清IL-10、IL-6、TNF-α表达的影响  

The Effect of Jianerqiangshen Electuary on Expression of Interleukin-10、Interleukin-6 and TNF-α on Asthmatic Guinea Pigs

作　　者：王禹斌[1] 朱顺星[2] 刘云[3] 刘春[2] 刘海鸥[2] 陈月明[4] 

机构地区：[1]苏州大学实验动物中心,江苏苏州215123 [2]南通大学实验动物中心,江苏南通226001 [3]南通市中医院儿科,江苏南通226001 [4]苏州市畜牧兽医站,江苏苏州215128

出　　处：《苏州大学学报（医学版）》2010年第4期728-731,897,共5页Suzhou University Journal of Medical Science

摘　　要：目的观察健儿强身膏对哮喘豚鼠血清IL-10、IL-6、TNF-α表达的影响。方法采用卵蛋白(OVA)致敏法制备豚鼠哮喘模型,将48只豚鼠随机分为6组,即空白对照组、模型组、阳性对照组、健儿强身膏(高、中、低)3个剂量组,观察各组引喘潜伏期(T)及肺组织的病理变化,应用酶联免疫吸附试验法测定血清IL-10、IL-6、TNF-α的表达水平。结果各治疗组均可延长引喘潜伏期,与模型组相比差异有统计学意义(P<0.05)。与空白对照组相比,模型组的IL-6、IL-10表达显著增加(P<0.01),TNF-α的表达无显著差异;各治疗组与模型组相比IL-6、IL-10表达显著下降(P<0.01),TNF-α的表达无显著差异。结论健儿强身膏能下调哮喘豚鼠IL-6、IL-10的表达,可能为其抑制哮喘呼吸道炎症的重要作用机制之一。Objective To observe the effect of Jianerqiangshen electuary on expression of IL-10,IL-6 and TNF-α on asthmatic guinea pigs.Methods The asthma guinea pig model was established by the ovalbumin（OVA） challenge methods.Forty-eight guinea pigs were randomly allocated to six groups：the blank control group,asthma model group,dexamethasone-treated group,three dose groups of Jianerqiangshen electuary.The effects of relieving asthmatic attacking were observed and the morphology structures were observed.The concentrations of IL-10,IL-6 and TNF-α were measured by sandwich enzyme-linked immunosorbent assay.Results Compared with the normal group,IL-6 and IL-10 of the model group significantly increase（P 〈0.05）.The IL-6 and IL-10 in guinea pigs were obviously lower than that of the asthmatic guinea pigs（P 〈0.01） after Jianerqiangshen electuary treatment,and TNF-α was not different between the groups treated respectively and the model groups.Conclusion Jianerqiangshen electuary can depress IL-6 and IL-10 expression,and it may be a key process in modulatory mechanism of asthma.

关 键 词：健儿强身膏 豚鼠 哮喘 IL-10 IL-6 TNF-Α 

分 类 号：R725.605[医药卫生—儿科]