八角莲水溶性有效成分的分离与抗病毒活性的测定  

Isolation of Effective Constituents from the Hydrophilic Fraction of Dysosma Versipellis and Their Antivirus Effect on CB_(1～6)V and HSV - 1 Virus

作　　者：姚莉韵[1] 王丽平[1] 

机构地区：[1]上海第二医科大学化学教研室,200025

出　　处：《上海第二医科大学学报》1999年第3期234-237,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金!39370831

摘　　要：目的研究八角莲Dysosmaverslpellis（Hance）M．Cheng．vartomentosa水溶液中抗病毒的有效成分。方法八角莲水溶液经提取分离，分得单体进行结构鉴定（UV、IR、1HNMR、EI－MS和13CNMR）及体外抗病毒试验。结果分到四个单体：Ⅰ.山奈酚-3-O-β-吡喃葡萄糖甙；Ⅱ.槲皮素-3－O-β-呋喃葡萄糖甙；Ⅲ．山奈酚；Ⅳ.苦鬼臼毒素。晶Ⅱ对HSV－1有效抑制浓度（CPEI50）为0．02μg／ml，晶Ⅲ、Ⅳ对CBV的CPEI50分别为1．90（B1.3）和3.12（B1.5）.6.25（B4）μg／ml；而对HSV－1的CPEI50分别为0．03μg／ml和5．00×10－4μg／ml．结论晶Ⅱ只对HSV－1有抑制作用，而晶Ⅲ.Ⅳ对CBV和HSV－1均有显著抑制作用。Objective To study effective constituents of antivirus from the hydrophilicfraction of Dysosma versiPellis. Methods The compounds were isolated from the hydrophi-lic fraction of Dysosma versipellis, which were characterized (through UV, IR, 1HNMR,EI - MS and 13CNMR) and determined their antivirus effect on CB1～6V and HSV - 1 virus invitro. ResuIts Four monomers were isolated: I. kaempferol - 3 - O -β - D - glucopy-ranoside, II. quercetin - 3 - O - β- D - glucofuran0side. Ⅲ. kaempferol Ⅳ. picropodophllin.The CPEI50 of II on HSV- 1 was 0.02μg/ml, the CPEI50 of III and that of IV on CBV viruswere l.90(B1,3)μg/ml and 3.12(B1.5)μg/ml,6.25(B4)μg/ml, respectively; while on HSV - 1 viruswere 0.O3μg/ml. 5.0OX 1O-4μg/ml, respectively. Conclusion Monomer II only had effectagainst HSV- 1 virus, but IIIand IV showed better activitiy to inhibit cytopathic effect ofCBV and HSV - 1 virus infection.

关 键 词：八角莲 抗病毒活性 山奈酚 苦鬼臼毒素 有效成分 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]