CD4^+T细胞在痢疾疫苗诱导小鼠肠粘膜免疫应答中的作用  

Role of CD4^+T cells in mucosal immune respones of mice after oral immunization with the bivalent Shigella vaccine candidates

作　　者：张亚东[1] 高杰英[1] 彭虹[1] 邢丽[1] 曾关富[1] 

机构地区：[1]军事医学科学院微生物流行病学研究所,北京100850

出　　处：《细胞与分子免疫学杂志》1999年第2期115-117,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：以本室构建的双价痢疾候选疫苗株ＦＳ５４１６ 作为口服免疫原，检测了小鼠ＰＰ 结（Ｐｅｙｅｒ ，ｓ ｐａｔｃｈｅｓ） 中Ｔ 淋巴细胞的体外增殖反应， 观察了腹腔注射抗Ｌ３Ｔ４ ｍ Ａｂ 对小鼠肠粘膜部位产生特异性Ｉｇ 分泌细胞的影响。结果表明：①小鼠口服免疫后，ＰＰ 结中的ＣＤ４ ＋ Ｔ 细胞可产生明显的特异性增殖反应：②选择性地删除ＣＤ４ ＋ Ｔ 细胞后，小鼠肠粘膜无抗原特异性Ｉｇ 分泌细胞产生。以上提示，小鼠肠粘膜对痢疾疫苗的免疫应答需要ＣＤ４ ＋ Ｔ 细胞的参与。After the mice were orally immunized with live attenuated bivalent hybrid strain FS-5416 of S.flexneri 2a and S.sonnei,the role of CD4+T cells in Peyer's patches was studied by T cell proliferation assay in vitro and Ig isotype specific BA ELISPOT assay following selective depletion of CD4+T cells with monoclonal anti-L3T4 mAb.The CD4+T cells in peyer's patches produced strong proliferation respones after oral immunization,and anti L3T4 mAb treatment weekly resulted in a significant decrease of the antigen specific lg producing cells compared with controls.These results indicate a important role of CD4+T cells in mucosal immune responses.

关 键 词：CD^+T细胞 增殖反应 痢疾疫苗 肠粘膜 免疫应答 

分 类 号：R392.12[医药卫生—免疫学]