外源性一氧化氮对大鼠肝脏抗氧化物酶和药物代谢酶活性的影响  

Effects of exogenous nitric oxide on the activities of antioxidant enzymes and drug metabolizing enzymes in rat liver 1

作　　者：申兆菊[1,2] 肖希龙[1,2] 周宗灿 

机构地区：[1]中国农业大学动物医学院 [2]北京医科大学公共卫生学院

出　　处：《中国药理学与毒理学杂志》1999年第2期145-148,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金

摘　　要：用亚硝基铁氰化钠（ＳＮＰ）作为ＮＯ生成前体，以０，１，２，４和８ｍｇ·ｋｇ－１ｉｐ给予大鼠，每日一次，连续５ｄ，来研究外源性ＮＯ对大鼠肝脏细胞色素Ｐ４５０，苯胺羟化酶（ＡＨ），谷胱甘肽Ｓ－转移酶（ＧＳＴ）等药物代谢酶和过氧化氢酶（Ｃａｔ），谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ），超氧化物歧化酶（ＳＯＤ）等抗氧化物酶活性以及脂质过氧化的体内影响情况．结果表明外源性ＮＯ能明显降低大鼠肝脏ＳＯＤ，Ｃａｔ，ＡＨ的活性和细胞色素Ｐ４５０的含量（Ｐ＜０．０５），并且高剂量组还能明显促进脂质过氧化发生（Ｐ＜０．０５）。This paper reports the effects of exogenous nitric oxide(NO) generated from sodium nitroprusside(SNP) on the activities of hepatic antioxidant enzymes such as catalase(Cat), glutathione peroxidase(GSH Px), superoxide dismutase(SOD) and metabolizing enzymes such as aniline hydroxylase(AH), glutathione S transferase(GST), cytochrome P450(P450) and lipid peroxidation in rats. Fifteen Wistar rats were randomly divided into five groups, each group of three rats was received SNP respectively at concentration of 0(control group), 1, 2, 4 and 8 mg·kg 1 body weight by intraperitoneal injection. As compared with the control group, the activities of Cat, SOD, AH and content of P450 in SNP treated groups were significantly reduced(P<0.05), and level of lipid peroxidation in the highest dose group was increased(P<0.05). The activities of GST and GSH Px of the SNP treated groups were not different from that of the control group. The results of this experiment provide an indication of toxicological effect of exogenous NO.

关 键 词：一氧化氮 硝普钠 肝脏 药物代谢酶 抗氧化物酶 

分 类 号：R969.1[医药卫生—药理学]