地塞米松及甘草酸对小鼠肝细胞凋亡的影响  

Effects of dexamethasone and glycyrrhizin on the hepatocyteapoptosis caused by phenobarbital withdrawal in mice

作　　者：李平[1] 陈东鸿[1] 程建峰[1] 仲崇华[1] 贺建荣[1] 滕树保[1] 贺平[1] 

机构地区：[1]第四军医大学唐都医院药学部

出　　处：《中国药理学通报》1999年第2期143-145,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金

摘　　要：目的研究地塞米松（ＤＭ）及甘草酸（ＧＬ）对小鼠肝细胞凋亡的影响。方法以肝／体重比、肝组织ＤＮＡ含量、组织学改变及原位凋亡细胞ＴＵＮＥＬ反应为指标，观察ＤＭ（９ｍｇ·ｋｇ－１）及ＧＬ（５０ｍｇ·ｋｇ－１），ｉｐ，每８ｈ１次，对撤除苯巴比妥（ＰＢ）后引起的小鼠肝细胞凋亡的影响。结果与ＰＢ不撤除对照组相比，ＤＭ组小鼠肝／体重比及肝ＤＮＡ含量无明显回落，组织学检查未见明显凋亡改变，ＴＵＮＥＬ反应阴性。ＧＬ组小鼠肝／体重比及ＤＮＡ含量分别回落１２０％和１３６％，肝细胞出现典型凋亡改变，ＴＵＮＥＬ反应阳性。结论ＤＭ能阻断撤除ＰＢ诱导的小鼠肝细胞凋亡，甘草酸对其无影响。AIM To investigate the effects of dexamethasone (DM) and glycyrrhizin (GL) on the hepatocyte apoptosis caused by phenobarbital (PB) withdrawal in mice. METHODS The ratio of liver/body weight, hepatic DNA content, morphological changes and TUNEL reaction (TdTmediated XdUTP nick labeling) as indices of evaluating hepatocyte apoptosis, were used to observe the effects of DM (9 mgkg-1, ip) and GL (50 mgkg-1, ip) on the hepatocyte apoptosis caused by PB withdrawal. RESUITS The regression of hepatic DNA content and ratio of liver/body weight and typical hepatocyte apoptosis, which occured in the control as mice after stopping PB treatment, were not found in the mice treated with DM, and a negative result of TUNEL assay was presented. However, in the mice given GL the ratio of liver/body weight and hepatic DNA content regressed by 120% and 136%, respectively; with a positive reaction of TUNEL assay and the typical changes of hepatocyte apoptosis. CONCLUSION The results showed that DM, but not GL, preve

关 键 词：地塞米松 甘草酸 苯巴比妥钠 肝细胞凋亡 肝疾病 

分 类 号：R975.5[医药卫生—药品] R575.05[医药卫生—药学]