HPLC法测定小儿肺热咳喘口服液中盐酸麻黄碱及盐酸伪麻黄碱的含量  

Determination of Ephedrine Hydrochloride and Pseudoephedrine Hydrochloride in Xiaoer Feire Kechuan Oral Solution by HPLC

作　　者：太成梅[1] 那微[1] 赵晓霞[1] 张清波[1] 

机构地区：[1]黑龙江省药品检验所,哈尔滨150001

出　　处：《中国药品标准》2010年第5期380-383,共4页Drug Standards of China

摘　　要：目的:建立小儿肺热咳喘口服液中盐酸麻黄碱及盐酸伪麻黄碱含量的HPLC测定法。采用Agilent XDB-C18柱,流动相为乙腈-0.2%磷酸(3:97),检测波长:210nm。结果:盐酸麻黄碱在0.03068～1.534μg(r=1.0000)范围内成线性关系,平均回收率(n=6)为98.6%,RSD=2.6%。盐酸伪麻黄碱在0.03110～1.555μg(r=1.0000)范围内成线性关系,平均回收率(n=6)为97.8%,RSD=2.4%。结论:本法简便、可靠、准确,可用于该制剂的质量控制。Objective： To establish an HPLC method of determining Ephedrine Hydrochloride and Pseudoephedrine Hydrochloride in Xiaoer Feire Kechuan Oral Solution. Methods：Agilent XDB-C18 column was used. The mobile phase was acetonitrile-0. 2% phosphoric acid （3：97 ） and The detection wavelength was 210 nm. Results：The linear range of Ephedrine Hydrochloride was 0. 030 68 - 1. 534 μg（ r = 1. 000 0）. The average recovery was 98. 6% with RSD 2. 6% （n = 6）. The linear range of Pseudoephedrine Hydrochloride was 0.031 10 - 1. 555μg（ r = 1. 000 0）. The average recovery was 97.8 % with RSD 2.4% （ n = 6 ）. Conclusion ： The method is reliable, accurate and can be applied to the quality control of the preparation.

关 键 词：高效液相色谱法 麻黄 盐酸庥黄碱 盐酸伪麻黄碱 

分 类 号：R287[医药卫生—中药学]