Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses： Potential and Challenges  

作　　者：张翀 邢新会 

机构地区：[1]Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

出　　处：《Chinese Journal of Chemical Engineering》2010年第5期863-869,共7页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China （20836004 20806046）; the Special Fund for Major State Basic Research Program of China （2009CB724702）; the National High Technology Research and Development Program ofChina （2009AA062903）

摘　　要：Green fluorescent protein (GFP) and its variants /homolog proteins are generally called as GFP-like fluorescent proteins (FPs), which are widely used as visible molecular tools for monitoring a wide range of biological processes due to their capability of simple, accurate and real time quantification. The FPs-based molecular and visible quantification tools are giving more impact on bioprocess engineering, enabling the biomolecule-level dynamic information to be linked with the process-level events. In this review, different applications of FPs in biological engineering with emphasis on rapid molecular bioprocess quantification, such as quantification of the transcription efficiency, the protein production, the protein folding efficiency, the cell concentration, the intracellular microenvironments and so on, would be first introduced. The challenges of using FPs with respect to actual bioprocess applications for the precise quantification including the interaction of FPs and the fused partner proteins, the maturation of FPs, the inner filter effect and sensing technology were then discussed. Finally, the future development for the FPs used in molecular bioprocess quantification would be proposed.Green fluorescent protein （GFP） and its variants /homolog proteins are generally called as GFP-like fluorescent proteins （FPs）, which are widely used as visible molecular tools for monitoring a wide range of biological processes due to their capability of simple, accurate and real time quantification. The FPs-based molecular and visible quantification tools are giving more impact on bioprocess engineering, enabling the biomolecule-level dynamic information to be linked with the process-level events. In this review, different applications of FPs in biological engineering with emphasis on rapid molecular bioprocess quantification, such as quantification of the transcription efficiency, the protein production, the protein folding efficiency, the cell concentration, the intracellular microenvironments and so on, would be first introduced. The challenges of using FPs with respect to actual bioprocess applications for the precise quantification including the interaction of FPs and the fused partner proteins, the maturation of FPs, the inner filter effect and sensing technology were then discussed. Finally, the future development for the FPs used in molecular bioprocess quantification would be proposed.

关 键 词：green fluorescent protein fluorescent proteins bioprocess engineering quantification MARKER 

分 类 号：Q78[生物学—分子生物学] Q436