Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125  

作　　者：李政 邓利 鲁吉珂 郭小雷 杨自信 谭天伟 

机构地区：[1]Beijing K(ey Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China [2]School of Textiles, Tianjin Polytechnic University, Tianjin 300160, China [3]Bioengineering Department, Zhengzhou University, Zhengzhou 450001, China

出　　处：《Chinese Journal of Chemical Engineering》2010年第5期870-875,共6页中国化学工程学报（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China (2006AA020101, 2007AA10Z360,2009AA03Z232);Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period (2008BA163B07)

摘　　要：The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were: 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were： 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.

关 键 词：immobilized lipase TRANSESTERIFICATION rice bran oil METHANOLYSIS optimization 

分 类 号：TQ423.2[化学工程] TQ225.241