中药材成分有机溶剂与离子液体分散液相微萃取方法的比较及其机理探讨  

Mechanism and Comparison of Dispersive Liquid Phase Microextraction Based on Organic Solvent and Ionic Liquid for Determination of Compounds in Traditional Chinese Medicine

作　　者：田杰[1] 陈璇[1] 白小红[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《分析化学》2010年第11期1593-1598,共6页Chinese Journal of Analytical Chemistry

基　　金：山西省自然科学基金(No.2007011086)资助项目

摘　　要：研究了离子液体分散液相微萃取(ILDLPME)机理;比较了ILDLPME和有机溶剂分散液相微萃取(OSDLPME)在测定蒽醌类化合物中的异同;建立了分散液相微萃取-高效液相色谱法测定药材中6种游离蒽醌类化合物(芦荟大黄素、大黄酸、丹蒽醌、大黄素、大黄酚和大黄素甲醚)含量的方法。在优化的实验条件下,OSDLPME和ILDLPME对6种分析物的富集倍数分别为101～230和76～181;6种分析物的检出限分别在20～200ng/L和40～400ng/L之间;精密度(RSD)分别在3.1%～10.0%和1.3%～7.0%之间;4种中药材中分析物的回收率在81.7%～110.7%和81.9%～110.8%之间。离子液体在水中分散的同时进行有序排列,形成分子有序组合体,对分析物进行萃取。ILDLPME达平衡时间更快,精密度更高,方法更简便;OSDLPME浓缩倍数更高,检出限更低;两种方法对中药样品中游离蒽醌类化合物含量测定结果无显著性差异。Mechanism of ionic liquid based dispersive liquid phase microextraction （ ILDLPME） has been investigated. The ILDLPME and organic solvent based dispersive liquid phase microextraction （ OSDLPME） have been critically compared for the determination of free anthraquinones in traditional Chinese medicines. Dispersive liquid phase microextraction （ DLPME） combined with HPLC has been developed for the determination of six free anthraquinones （ aloe-emodin,rhein,danthron,emodin,chrysophanol and physcione） . Under the optimal conditions,the enrichment factors （ EFs） were in the range of 101 230 and 76 181 for OSDLPME and ILDLPME respectively. The limits of detection were 20 200 ng/L and 40 400 ng/L; the relative standard deviations （ RSDs,n = 3） were 3. 1% 10. 0% and 1. 3% 7. 0% ; the recoveries of the analytes in four kinds of traditional Chinese medicines were 81. 7% 110. 7% and 81. 9% 110. 8% for OSDLPME and ILDLPME combined with HPLC,respectively. Ionic liquid was orderly arranged and molecular-ordered organized assembly formed,when ionic liquid dispersed in sample solution,then the analytes were extracted. Compared with OSDLPME,the ILDLPME was more effective and more convenient,the repeatability of ILDLPME was better than that of OSDLPME. However,higher enrichment factors and lower limits of detection could be obtained by OSDLPME. There were no significant deviations between two methods for the determination of free anthraquinones.

关 键 词：分散液相微萃取 离子液体 游离蒽醌类化合物 分子有序组合体 

分 类 号：TQ461[化学工程—制药化工]