基于聚酰胺6纳米纤维膜的固相萃取-高效液相色谱法测定兔血浆中酮康唑对映体  

Determination of Ketoconazole Enantiomers in Rabbit Plasma Samples Using Polyamide 6 Nanofibers Mat-based Solid Phase Extraction Coupled with Liquid Chromatography

作　　者：王敏[1,2] 许茜[1,3,2] 殷雪琰[1,2] 张妮萍[1,2] 申艳艳[1,2] 汪海峰[4] 顾忠泽[3,2] 

机构地区：[1]东南大学环境与医学工程教育部重点实验室,南京210009 [2]东南大学苏州市环境与生物安全重点实验室,苏州215123 [3]东南大学生物电子学国家重点实验室,南京210096 [4]南京财经大学食品科学与工程学院,南京210003

出　　处：《分析化学》2010年第11期1604-1608,共5页Chinese Journal of Analytical Chemistry

基　　金：东南大学国家自然科学基金项目预研基金(No.9225000007);苏州市科技局科技发展计划(No.YJS0948)资助项目

摘　　要：以基于聚酰胺6纳米纤维膜的固相萃取法,结合高效液相色谱手性流动相添加剂法测定了兔血浆中的酮康唑对映体的浓度。仅用1.5mg聚酰胺6纳米纤维膜、100μL甲醇即可完成目标物的富集和洗脱。酮康唑的2个对映体在50.0～400.0μg/L范围内呈良好的线性;方法的定量限为40.0μg/L;日内和日间精密度分别小于6.3%和8.6%;平均绝对回收率为79.4%～85.6%;平均相对回收率为90.0%～96.5%。本方法灵敏、准确、重现性好,符合生物样本中酮康唑对映体分析测定的要求。A method was developed to determine ketoconazole enantiomers in rabbit plasma by polyamide 6 nanofibers mat-based solid phase extraction and chiral mobile phase additive method following high-performance liquid chromatography. Only 1. 5 mg of polyamide 6 nanofibers membrane and 100 μL methanol can complete the enrichment and the elution of the target material. Calibration curves were linear over the range of 50. 0 400. 0 μg/L for each enantiomer. The limit of quantitation was 40. 0 μg/L for each enantiomer. The intra-day and inter-day relative standard deviation was 4. 3% and 7. 6% ,respectively. The average absolute recoveries and relative recoveries were 79. 4% 85. 6% and 98. 0% 102. 2% ,respectively. This method is sensitive,accurate,reproducible,and can meet the requirements of the determination of ketoconazole enantiomers in biological samples.

关 键 词：酮康唑 对映体 聚酰胺6纳米纤维膜 固相萃取 高效液相色谱 

分 类 号：R96[医药卫生—药理学]