基于双氢青蒿素-巯基化合物电化学探针对巯基化合物的检测  

Detection of Sulfhydryl Compounds Based on Dihydroartemisinin-Sulfhydryl Compounds Complex Electrochemical Probe

作　　者：王辉[1] 杨培慧[1] 黄连喜[1] 蔡继业[1] 

机构地区：[1]暨南大学化学系,光电信息与传感技术广东普通高校重点实验室(暨南大学),广州510632

出　　处：《分析化学》2010年第11期1647-1651,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.30828028,30872404)资助项目

摘　　要：在20%乙醇+B-R缓冲溶液(pH7.2)介质中,利用双氢青蒿素与谷胱甘肽、6-巯基嘌呤及L-半胱氨酸作用,分别于-1.05,-1.20和-1.10V处获得双氢青蒿素-巯基化合物复合物灵敏的还原峰,该还原峰在一定浓度范围内随着巯基化合物以及双氢青蒿素浓度的增加而增大,因此可将其作为电化学探针应用于上述3种巯基化合物以及双氢青蒿素的检测,为检测巯基化合物提供了一种新方法;探讨了复合物形成的电极反应机理,利用纳米金与巯基的共价作用阻断巯基参与形成复合物,证实了复合物是由双氢青蒿素中的碳自由基与巯基化合物巯基中的硫原子通过SC键结合形成。In B-R buffer solution containing 20% ethanol（ pH 7. 2） ,the sulfhydryl compounds（ RSH） ,such as glutathione,6-mercaptopurine and L-cysteine,bound with dihydroartemisinin （ DHA） and the RSH-DHA complex was formed on electrode surface through cathodic scanning-mediated interaction. The sensitive reduction peaks of the complex were obtained at -1. 05,-1. 20 and -1. 10 V,respectively. There are good responses between peak current and the concentrations of DHA or RSH. The binding mechanisms of DHA with RSH were also discussed. In addition,gold nanoparticles were used to block the binding site of sulfhydryl-DHA by the strong covalent bond of S-Au. It confirmed that the DHA-RSH complex was formed through the interaction between C-centered free radical derived from dihydroartemisinin and S-atom from sulfhydryl compounds.

关 键 词：巯基化合物 双氢青蒿素 复合物 电化学探针 

分 类 号：R96[医药卫生—药理学]