液相色谱-串联质谱法同时测定7种抗抑郁类和5种抗精神病类药物的血药浓度  

Quantitative determination of seven antidepressants and five antipsychotics in human serum by HPLC-MS/MS

作　　者：贾晶莹[1] 张梦琪[1] 桂雨舟 刘罡一[1] 陆川[1] 李水军[1] 余琛[1] 

机构地区：[1]上海市徐汇区中心医院中心实验室,上海200031 [2]上海医药临床研究中心分析化学部,上海200233

出　　处：《中国药物应用与监测》2010年第5期272-275,共4页Chinese Journal of Drug Application and Monitoring

基　　金：上海市科委科研计划项目(08411966700)

摘　　要：目的：建立同时测定血清中7种抗抑郁类药物和5种抗精神病类药物浓度的液相色谱-串联质谱（HPLC-MS/MS）方法。方法：血清样品中加入还阳酚（内标,西地那非中间产物）,采用乙腈直接沉淀法处理样品。分析柱为XbridgeTMPhenyl柱（150mm×2.1mm,5μm）;流动相为含0.02%甲酸水溶液-含0.02%甲酸乙腈溶液（35：65）,等度洗脱,流速为0.3mL·min-1,进样量为20μL。采用电喷雾离子源,多离子反应监测（MRM）,正离子扫描进行测定。结果：7种抗抑郁类药物和5种抗精神病类药物线性范围在2~1000μg·L-1,线性相关系数均大于0.9931,各浓度的批内和批间RSD〈10%,提取回收率为102%~112%,方法准确度为89.3%~107.9%。结论：本方法操作简便,特异性强,灵敏度高,可同时进行7种抗抑郁类药物和5种抗精神病类药物的血药浓度监测。Objective：To establish an HPLC-MS/MS method for determination of seven antidepressants and five antipsychotics in human serum.Methods：The serum sample was precipitated with acetonitrile after the addition of internal standard（huanyangfen）.The chromatographic separation was performed on XbridgeTM Phenyl column（150 mm×2.1 mm,5 μm） with the mobile phase of 0.02% formic acid water-0.02% formic acid acetonitrile（35：65） at a flow rate of 0.3 mL·min-1.The injection volume was 20 μL.The deprotonated ion of analyte was ionized by turbo ion spray and was detected in positive ionization by multiple reaction monitoring mode（MRM）.Results：The linear concentration ranges of the calibration curves for seven antidepressants and five antipsychotics were 2-1000 μg·L-1.The coefficients of linearity were more than 0.993 1.RSDs of withinbatch and between-batch were less than 10%.The average recovery ranged from 102% to 112%.The accuracy ranged from 89.3% to 107.9%.Conclusion：The method is simple and quick with high specificity and sensitivity,and it is suitable for quantitative determination of seven antidepressants and five antipsychotics in human serum.

关 键 词：液相色谱-串联质谱法 血清 血药浓度 五氟利多 匹莫奇特 氯普噻吨 硫利达嗪 三氟拉嗪 阿米替林 氯米帕明 氟西汀 丙咪嗪 马普替林 地昔帕明 舍曲林 

分 类 号：R96[医药卫生—药理学]