慢性乙型肝炎患者血清中HBeAg、HBsAg与HBV-DNA的关系  

The relationship between serum HBeAg,HBsAg and HBV-DNA in the chronic hepatitis B patients

作　　者：徐敬轩[1] 谢而付[2] 黄珮珺[2] 陈丹[2] 戎国栋[2] 

机构地区：[1]江苏省丹阳市第二人民医院检验科,212300 [2]南京医科大学第一附属医院检验科,210029

出　　处：《检验医学与临床》2010年第21期2337-2338,2340,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨慢性乙型肝炎患者血清中HBeAg、HBsAg与乙肝病毒HBV-DNA之间的关系。方法选取655例HBsAg阳性的慢性乙型肝炎患者血清标本,用微粒子酶免疫分析法(MEIA)检测HBeAg、HBsAg,用荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA。结果在HBeAg阳性标本中,HBV-DNA阳性率为64.64%;HBeAg阴性标本中,HBV-DNA阳性率为36.61%,二者差异有统计学意义(P<0.01)。不同浓度组HBeAg和HBV-DNA检测结果比较,差异有统计学意义(P<0.01)。不同载量HBV-DNA组,HBeAg差异有统计学意义(P<0.01),且随HBV-DNA含量增加而增加;HBsAg差异也有统计学意义(P<0.01),但随HBV-DNA含量增加而降低。结论同时检测HBV-DNA与HBeAg能更好地用于临床诊断与疗效观察,HBeAg随着HBV-DNA拷贝数的增加而增加,而HBsAg却呈下降趋势。Objective To explore the relationship between serum HBeAg,HBsAg and HBV-DNA concentration.Methods The serum samples of 655 chronic hepatitis B patients who showed HBsAg positive were collected.The HBeAg and HBsAg were detected by micro particle enzyme immunoassays（MEIA）and HBV-DNA was tested by fluorescence quantitative polymerase chain reaction（FQ-PCR）.Results In the HBeAg positive samples,the positive percentage of HBV-DNA was 66.64%,and in the HBeAg negative samples,the positive percentage of HBV-DNA was 36.61%,which showed a significant difference（P〈0.01）between the two groups.The concentration of HBV-DNA was also of significant difference between the two groups（P〈0.01）.In the different concentration of HBV-DNA groups,both HBeAg and HBsAg show significant difference（P〈0.01）.With the increase of HBV-DNA,the HBeAg was increasing,but HBsAg was decreasing.Conclusion The joint test of HBeAg and HBV-DNA was a better way to detect the replication of HBV,which would be helpful for the HBV clinical therapy.With the increase of HBV-DNA,the HBeAg was increasing,but HBsAg was decreasing.

关 键 词：慢性乙型肝炎 乙型肝炎病毒表面抗原 乙型肝炎病毒E抗原 HBV-DNA 

分 类 号：R446.1[医药卫生—诊断学]