GDNF和IL-1β体外诱导中脑神经干细胞分化的实验研究  

Experimental Study on Differentiation of Mesencephalic Neural Stem Cells Induced by GDNF and IL-1β in Vitro

作　　者：丁文杰[1] 丁继固[2] 李光[3] 

机构地区：[1]武汉大学基础医学院,研究生湖北武汉430071 [2]咸宁学院基础医学院解剖学教研室 [3]武汉大学中南医院ICU

出　　处：《咸宁学院学报（医学版）》2010年第5期369-373,共5页Journal of Xianning Univarsity(medical Sciences)

摘　　要：目的探索胶质源性神经营养因子(GDNF)和白细胞介素1β(IL-1β)在体外诱导小鼠胚胎中脑神经干细胞(M-NSCs)分化成多巴胺能神经元的培养方法,为M-NSCs移植治疗帕金森病(PD)提供实验依据。方法在有血清条件下体外培养鼠胚M-NSCs,予以GDNF和IL-1β作诱导分化。TH免疫细胞化学鉴定,流式细胞术检测TH阳性神经元的百分率。结果实验组促进M-NSCs分化为TH阳性神经元的比例:A组为13.53%,B组为9.66%,C组为16.22%,D组(对照组)仅3.46%,有显著性差异(P<0.01)。结论 GDNF和IL-1β可明显促进M-NSCs分化成多巴胺能神经元。Objective To explore the culture methods for the differentiation of mesencephalic neural stem cells（M-NSCs） from embryonic mice into dopaminergic neurons in vitro,and to provide the experimental information for the therapy of Parkinson＇s disease by cell transplantation. Methods M-NSCs were cultured and proliferated,then GDNF and IL-1β were used to induce differentiation in the condition of fetal bovine serum（FBS）.Immunocytochemical technique was used to detect the TH-ir cells from NSC differentiation,and FCM was used to detect the percentage of TH-ir in all the cells.Results The proportions of M-NSCs differentiation into TH positive cells were 13.53% in group A,9.66% in group B,16.22% in group C,respectively,and were much higher than that in control group（3.46%）.There were significant differences between each experimental group and control group（P0.01）.Conclusion GDNF and IL-1β can significantly increase the differentiation of dopaminergic neurons.

关 键 词：中脑神经干细胞 多巴胺能神经元 胶质源性神经营养因子 白细胞介素-1β 酪氨酸羟化酶 

分 类 号：R965.1[医药卫生—药理学]