云南省白族六个基因座的遗传多态性调查  

Distribution of six STR loci in Bai ethnic group in Yunnan province of China

作　　者：邹苹 杨燕 李德林[1,2,3,4] 邹浪萍 申滨[1,2,3,4] 

机构地区：[1]昆明市妇幼保健医院 [2]云南省人民医院 [3]云南省高级人民法院司法鉴定科学技术研究所 [4]昆明医学院

出　　处：《中华医学遗传学杂志》1999年第3期160-163,共4页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金

摘　　要：目的了解云南省白族６个短串联重复序列基因座遗传多态性分布。方法应用复合扩增技术对ＣＳＦ１ＰＯ、ＴＰＯＸ、ＴＨ０１、Ｆ１３Ａ０１、ＦＥＳＦＰＳ和ｖＷＡ等６个ＳＴＲ基因座进行分析，采用高分辨率的聚丙烯酰胺凝胶电泳分离、银染法显影技术，对云南白族上述６个ＳＴＲ基因座进行遗传多态性调查。结果ＣＳＦ１ＰＯ基因座，观察到７个等位基因，１９个基因型；ＴＰＯＸ基因座，观察到５个等位基因，１１个基因型；ＴＨ０１基因座，观察到６个等位基因，１６个基因型；Ｆ１３Ａ０１基因座，观察到６个等位基因，１６个基因型；ＦＥＳＦＰＳ基因座，观察到７个等位基因，１５个基因型；ｖＷＡ基因座，观察到７个等位基因，２１个基因型。结论上述６个短串联重复序列基因座基因频率分布与Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡吻合良好。Objective This study was conducted to make known the distribution of six STR loci in Bai ethnic group in Yunnan province. Methods DNA extraction from blood samples (126 in number) and multiplex amplification of CSF1PO, TPOX, TH01, F13A01, FESFPS and vWA were carried out. Using denaturing polyacrylamide gel electrophoresis and silver stain, the authors investigated the distribution of allele frequencies of CSF1PO, TPOX, TH01, F13A01, FESFPS and vWA loci in Bai ethnic group living in Yunnan province. Results 7 alleles and 19 genotypes of CSF1PO locus, 5 alleles and 11 genotypes of TPOX locus, 6 alleles and 16 genotypes of TH01 locus, 6 alleles and 16 genotypes of F13A01 locus, and 7 alleles and 15 genotypes of FESFPS locus,and 7 alleles and 21 genotypes of vWA locus were observed. Conclusion The allele distribution of the loci was in good agreement with the HardyWeinberg equilibrium.

关 键 词：云南 白族 CSF1PO基因座 TPOX基因座 遗传多态性 

分 类 号：Q346.5[生物学—遗传学] Q986