大戟狼毒对朱砂叶螨的毒性作用  

Acarocidal Actions of Euphorbia fischeriana against Tetranychus cinnabarinus

作　　者：刘素琪[1] 顾雅静[1] 王海香[2] 董海龙[1] 师光禄[3] 曹挥[1] 

机构地区：[1]山西农业大学农学院,太谷030801 [2]山西农业大学林学院,太谷030801 [3]北京市农业应用新技术重点实验室,北京102206

出　　处：《林业科学》2010年第10期91-94,共4页Scientia Silvae Sinicae

基　　金：山西省青年科学基金资助项目(20051036;2006021033)

摘　　要：研究大戟狼毒石油醚提取物中最佳杀螨活性流分11(EFPE-11)处理雌成螨后的症状表现,并采用生化方法测定EFPE-11对朱砂叶螨体内的蛋白含量、谷胱甘肽-S-转移酶、乙酰胆碱酯酶和Ca2+-Mg2+ATP酶活性的影响。从症状观察初步推测它们可能通过影响朱砂叶螨的神经传导而导致雌成螨死亡。EFPE-11击倒作用迅速,从12h开始朱砂叶螨就进入了大量死亡期。在施药过程中,EFPE-11引起成螨体内谷胱甘肽-S-转移酶的活性被激活,而乙酰胆碱酯酶活性则被一定程度的抑制,Ca2+-Mg2+ATP酶活性最后被强烈抑制。乙酰胆碱酯酶和Ca2+-Mg2+ATP酶的被抑制,会造成神经传递的阻断,导致螨体的死亡,而神经传递的阻断,可能是EFPE-11作用螨体的主要方式。The fraction 11（EFPE-11） extract of Euphorbia fischeriana with petroleum ether was tested for bio-activity and mechanisms,including the effect on the protein content,the activities of GSTs,AchE and Ca^2 ＋-Mg^2＋ ATPase of Tetranychus cinnabarinus. The symptomatic expression and the results of the action mechanism indicated that EFPE-11 could interrupt the nerve transmission of mites. The survival rate of mites began to decrease quickly when treated with EFPE-11 in 12 h. The activity of GSTs in treated mites was activated,but the activity of acetyl cholinesterase（AchE） was inhibited. The activity of Ca^2 ＋-Mg^2＋ ATPase was strongly inhibited. The inhibition of the AchE and Ca^2 ＋-Mg^2＋ ATPase activity can cause interruption of the nerve transmission and eventually cause mite death. The results suggested that the interruption of the nerve conduction could be the main reason for EFPE-11 against mites.

关 键 词：大戟狼毒 朱砂叶螨 作用机制 

分 类 号：S767.3[农业科学—森林保护学]