萹蓄提取物对朱砂叶螨的触杀活性及相关酶活性的影响  

Acaricidal Activities of Polygonum aviculare Extracts against Tetranychus cinnabarinus and Their Effects on Enzyme Activities in the Mite

作　　者：王有年[1] 李青[1,2] 李照会[2] 师光禄[1] 

机构地区：[1]北京农学院生物技术系农业部都市农业(北方)重点开放实验室北京农业应用新技术重点实验室,北京102206 [2]山东农业大学植保学院,泰安271018

出　　处：《林业科学》2010年第10期103-107,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30872029);北京市自然科学基金重点项目(6071001);北京市自然科学基金项目(6092007);北京市科委区县专项资金项目;北京市教委平台建设项目;北京市都市农业学科群项目(XK100190553);北京市属高校人才强教深化计划资助项目(PHR20090516;PHR200906134)

摘　　要：研究萹蓄不同溶剂提取物对朱砂叶螨的毒力,发现萹蓄氯仿提取物的杀螨活性最高,浓度为2mg·mL-1时,雌成螨48h的校正死亡率可达89.95%,LC50为(0.7153±0.1253)mg·mL-1,明显高于石油醚、甲醇提取物(P<0.05)。对萹蓄氯仿提取物进一步萃取、柱层析分离,发现最终得到的19个流分中,第2,7,8,15,16,17,18流分杀螨效果较好,死亡率均达到70%以上,其中流分16的杀螨率达到87.12%。采用比色法测定第16流分对几种酶活性的影响,发现其对朱砂叶螨乙酰胆碱酯酶(AchE),Na+、K+-ATPase,GSH-S-转移酶的活性均产生不同程度的影响。This paper showed a study on acaricidal activities of the plant extracts from Polygonum aviculare against Tetranychus cinnabarinus and the effects on the activities of several relevant enzymes. The plant extracts were extracted using different solvents such as chloroform,petroleum ether and methanol. It was found that the corrected mortality of female adults could reach 89. 95%（2 mg·mL^-1） and the half lethal concentration（LC50） was（0. 715 3 ± 0. 125 3） mg·mL^-1 when using chloroform as the solvent,which exhibited best acaricidal activities among three solvent extracts（P 0. 05） . Through further extraction and column chromatography,we obtained 19 components from the chloroform extracts,and No. 2,7,8,15,16,17 and 18 components had acaricidal activities higher than 70%,among them,No. 16 caused the mites the highest motality（87. 12%） . Furthermore,colorimetry assay showed that the component No. 16 could affect the activity of acetylcholinesterase（AchE） ,Na^ ＋,K ^＋-ATPase,GSH-S-transferase（GSTs） of T. cinnabarinus.

关 键 词：朱砂叶螨 萹蓄 乙酰胆碱酯酶 Na+ K+-ATPase GSH-S-转移酶 

分 类 号：S767.3[农业科学—森林保护学]