复方氯胺酮对恐惧幼鼠脑杏仁核NR2bR和GABAaR蛋白表达的影响  

Effect of compound ketamine oral solution on NR2b and GABAaR in amygdala , neurons of immature rats in fear conditions.

作　　者：刘文义[1] 臧瓅娜[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266000

出　　处：《中外医学研究》2010年第21期3-5,共3页CHINESE AND FOREIGN MEDICAL RESEARCH

摘　　要：目的 观察复方氯胺酮口服液对幼鼠的恐惧行为反应和大脑杏仁核甲基-D-天冬氨酸2b受体（N-methyl-D-aspartate 2B Receptor, NR2bR）和γ-氨基丁酸a受体（γ-aminobutyric acid A Receptor, GABAaR）蛋白表达影响.方法 用木僵行为实验对幼鼠恐惧状态下的行为学变化进行评分,用免疫组织化学方法检测复方氯胺酮口服液对在恐惧伤害性刺激下的幼鼠的大脑杏仁核NR2b和GABAa受体受体蛋白表达的影响.结果 模型组与对照组比较,木僵反应评分明显增加（P〈0.01）,GABAaR的表达增加（P〈0.05）,NR2b的表达减少（P〈0.05）;各用药组与模型组比较,木僵反应评分明显降低,复方氯胺酮组（CKOS组）和咪达唑仑组（M组）的GABAa的表达增加,CKOS组和氯胺酮组（K组）的NR2b的表达减少;各用药组之间的比较,木僵反应评分CKOS组高于M组,M组高于K组,GABAa的表达中CKOS组高于M组,NR2b的表达中CKOS组低于K组.结论 复方氯胺酮口服液具有抑制幼鼠恐惧条件反射产生的作用.Objective To observe the effect of compound ketamine oral solution on N - methyl - D - aspartate 2b receptor （ NR2b）, gamma - aminobutyric acid A receptors （ GABAAR ） in amygdala neurons and the behavioral response of immature rats in fear conditions. Methods Freezing Behavior were used to evaluate the behavioral response of immature rats in fear conditions and immune histochemistrstaining were used to check the expression of NR2bR and GABAaR in amygdala neurons of immature rats. Results Comparing control group and model group, freezing was signifcantly increased（P 〈0. 01 ）, the expression of GABAaR increased（P 〈0. 05 ） and expression of NR2bR decreased（P 〈0. 05）. As compared with model group, freezing was significantly decreased in CKOS group, K group and M group, the expression of GABAaR increased in CKOS and M goups,and the expression of NR2bR decreased in CKOS and K goups. Between CKOS,Kand M groups,the score of freezing of CKOS was highest, and that of M group higher than that of K group. In the 3 groups , the expression of GABAaR of CKOS group was significantly more than that of M group, however, the expression of NR2bR of CKOS group was significantly less than that of K group. Conclusion CKOS can inhibit conditioned fear of young lab mice. γaminobutyric acid （GABA） A receptor.

关 键 词：复方氯胺酮口服液 恐惧条件反射 甲基-D-天冬氨酸2b受体 γ氨基丁酸a受体 

分 类 号：R614[医药卫生—麻醉学]