ADS系列大孔吸附树脂纯化山楂叶总黄酮工艺研究  

Purification process for flavonoids from hawthorn leaves with ADS type of macroporous resin

作　　者：董恒颖[1] 王莹[1] 

机构地区：[1]天津华立达生物工程有限公司,天津300457

出　　处：《天津药学》2010年第5期67-71,共5页Tianjin Pharmacy

摘　　要：目的:研究ADS系列大孔吸附树脂分离纯化山楂叶总黄酮的工艺条件及参数。方法:以树脂对山楂叶总黄酮的吸附量和洗脱率为指标,对ADS系列大孔吸附树脂分离纯化山楂叶总黄酮的工艺条件进行筛选。结果:ADS-8型大孔吸附树脂对山楂叶总黄酮有较好的吸附分离性能,该树脂分离纯化山楂叶总黄酮的最佳工艺条件为:上柱液pH值4.5,上柱液总黄酮含量为1 000 mg/L,以流速3 BV/h上柱,上样量为100 ml(约5 BV);所用洗脱剂乙醇体积分数为40%,以2BV/h的流速洗脱,洗脱剂用量为150 m l(约7.5 BV)。经过上述工艺纯化后,所得产品总黄酮含量达到80%。结论:ADS-8型大孔吸附树脂适于分离纯化山楂叶总黄酮。Objective：To investigate the technical parameters of purification process of total flavonoids from hawthorn leaves with ADS type of macroporous resin.Methods：The conditions of purification of total flavonoids from hawthorn leaves were screened with adsorbing capacity and desorption rate of total flavonoids as indicators.Results：Among six types of macroporous adsorption resin choosed,ADS-8 is the best resin for separating and purifying of flavonoids from hawthorn leaves.The optimum conditions are：the concentration of hawthorn leaves flavonoids is 1 000 mg/L at the pH value of 4.5,flow velocity is 3 BV/h,the treated amount is 100 ml（about 5 BV）,the concentration of eluent is 40%（volume fraction） ethanol with 150 ml（about 7.5 BV）,and the flow velocity of eluent is 2 BV/h.After purifying by ADS-8,the purity of product can reach 80%.Conclusion：ADS-8 can be used in purification of total flavonoids of hawthorn leaves.

关 键 词：山楂叶 总黄酮 大孔吸附树脂 

分 类 号：R917[医药卫生—药物分析学]