纤维型和原浆型星形胶质细胞的分离培养及睫状神经营养因子对其增殖的影响  

Culture of processbearing and flat astrocytes in vitro and the proliferating effect of CNTF on them

作　　者：咸海青[1,2] 丁爱石[1,2] 梁平 汪家政[1,2] 邱宗荫[1,2] 范明 

机构地区：[1]军事医学科学院基础医学研究所 [2]重庆医科大学

出　　处：《军事医学科学院院刊》1999年第2期87-89,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的和方法：建立纤维型及原浆型星形胶质细胞体外分离培养的方法，并观察在体外睫状神经营养因子（ｃｉｌｉａｒｙｎｅｕｒｏｔｒｏｐｈｉｃｆａｃｔｏｒ，ＣＮＴＦ）对这两种细胞的增殖作用。结果和结论：经免疫组织化学鉴定，本方法分离培养的星形胶质细胞，纤维型纯度达９５％，原浆型纯度达９９％。ＣＮＴＦ可以促进纤维型星形胶质细胞的增殖，而对原浆型星形胶质细胞的促增殖作用不明显。Objective and Methods: To observe the proliferating effect of ciliary neurotrophic factor (CNTF) on processbearing and flat astrocytes using method of separation and culture. Results and Conclusion: Identified by immunocytochemistry, the purity of flat astrocytes after separation and culture was 99%, the purity of processbearing ones was 95%. CNTF promoted the proliferation of processbearing astrocytes, but not flat ones.

关 键 词：睫状神经 纤维型 星形胶质细胞 原浆型 增殖 

分 类 号：Q28[生物学—细胞生物学]