16SrRNA基因在新生儿败血症诊断中的应用  

Application of 16SrRNA gene in diagnosis of neonatal septicemia

作　　者：张勤勤[1] 龚华[1] 吴高雄[1] 

机构地区：[1]江苏省无锡市妇幼保健院检验科,214002

出　　处：《中国妇幼保健》2010年第31期4591-4592,共2页Maternal and Child Health Care of China

摘　　要：目的:应用聚合酶链反应(PCR)检测细菌16SrRNA基因,研究其在新生儿败血症中的应用。方法:抽取临床疑为细菌感染的265例新生儿的静脉血,分别做血培养和细菌16SrRNA的基因检测。于血标本中抽提DNA后用聚合酶链反应(PCR)扩增,将扩增产物电泳后经凝胶成像系统扫描。结果:PCR检测阳性率为6.4%(17/265),明显高于血培养的阳性率〔3.4%(9/265)〕,差异有统计学意义(P<0.05)。结论:与血培养相比,检测细菌16SrRNA基因对新生儿败血症诊断的特异性和敏感度更高。Objective：To investigate the application of 16SrRNA gene in diagnosis of neonatal septicemia by polymerase chain reaction（PCR）.Methods：Blood specimens from 265 neonates suspected of bacterial infection were cultured and bacterial 16SrRNA gene detection was conducted.DNA isolated from blood specimens was amplified by PCR and the results were analyzed by gel imaging system after gel electrophoresis.Results：The positive detection rate of PCR was 6.4%（17/265）,which was significantly higher than that of blood culture [3.4%（9/256）]（P0.05）.Conclusion：Compared with blood culture,the specificity and sensitivity of bacterial 16SrRNA gene detection are higher in diagnosis of neonatal septicemia.

关 键 词：新生儿败血症 16SrRNA 聚合酶链反应 

分 类 号：R722.131[医药卫生—儿科]