酸性染料比色法测定莱菔子中总生物碱的含量  

Determination of the Total Alkaloids in Semen Raphani by Acid Dye Colorimetry

作　　者：李彦博[1] 孙静[2] 

机构地区：[1]山东大学附属省立医院,山东济南250021 [2]山东中医药大学,山东济南250355

出　　处：《中医药信息》2010年第5期8-10,共3页Information on Traditional Chinese Medicine

基　　金：国家"十五"科技攻关计划课题(2001BA701A55-9)

摘　　要：目的：探讨莱菔子中总生物碱含量测定的方法。方法：采用酸性染料比色法测定莱菔子中总生物碱含量。结果：酸性染料比色法显色的条件为pH4.2的缓冲液10mL,0.04%的溴甲酚绿溶液2.0mL,氯仿萃取4次,每次5mL,检测波长332nm。芥子碱硫氰酸盐在1.696~16.960ug.mL-1范围内,与吸光度呈良好的线性关系,平均回收率99.75%,RSD为1.16%（n=6）。结论：该方法灵敏度高,准确性、重现性好。Objective： To investigate the method of determination of total alkaloids in Semen Raphani.Methods： The Contents of total alkaloids were determined by Acid dye colorimetry.Results： Acid dye color conditions： PH4.2 buffer 10mL,0.04% bromocresol green liquid 2.0mL,chloroform extraction four times,each time use 5ml,and the wavelength was at 332 nm.The linear range of Sinapine thiocyanate was 1.696～16.960μg/mL,the mean recovery was 99.75%,RSD（%）=1.16（n=6）.Conclusion： This method is simple,sensitive and accurate with good reproducibility.

关 键 词：酸性染料比色法 莱菔子 总生物碱 含量测定 

分 类 号：R284.1[医药卫生—中药学]