西洋参干预胰岛素抵抗大鼠活性部位的化学成分研究Ⅱ  

Studies on Chemical Constituents of Active Site that American Ginseng Impact Rat Insulin Resistance Ⅱ

作　　者：李冀[1] 马育轩[1] 葛鹏玲[1] 杨炳友[1] 尚广巍[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2010年第5期23-26,共4页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30672632);黑龙江省杰出青年科学基金项目(JC04-01);黑龙江省博士后科学基金(LRB09-321);黑龙江省留学回国人员科技项目择优资助基金

摘　　要：目的:研究西洋参干预胰岛素抵抗大鼠活性部位的化学成分。方法:以乙醇提取,用大孔树脂吸附,乙醇洗脱得总皂苷,再用硅胶柱色谱及HPLC等分离技术与方法分离和纯化,通过理化常数及光谱数据确定其结构。结果:共分得3个化合物,经鉴定后分别证明为:6-O-[α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖基]-20(24)-环氧-3,β6,α12,β25-四醇(Ⅰ);6-O-[α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖基]-20-O-β-D吡喃葡萄糖基-达玛-24-烯-3,β6α,12,β20S-四醇(Ⅱ);(14S)-3-(4,5-二羟-6-(羟甲基)-3-(3,4,5-三羟基-6-(羟甲基)-顺-4-四氢吡喃-2-氧)-顺-4-四氢吡喃-2-氧)-4,4,8,10,14-五甲基-17-((S)-6-甲基-2-(3,4,5-三羟基-6-((3,4,5-三羟基-6-羟甲基)-顺-4-四氢吡喃-2-氧)甲基)-顺-4-四氢吡喃-2-氧)庚-5-烯-2-基)-2,3,4,5,6,7,8,9,10,11,14,15,16,17-十四氢-1H-环戊烯并[a]菲2-醋酸酯(Ⅲ)。Objective：To study the chemical constituents of active site that American ginseng treats rat insulin resistance.Mefthods：They were extracted by ethanol and adsorbed by macro-reticular resins,then eluted with ethanol and isolated by silica gel column chromatography and HPLC preparation chromatography.Their structures were determined by physical and chemical properties,as well as spectrographical identification.Results：Three compounds were isolated from the active site that American ginseng treats rat insulin resistance.Their structures were identified as 6-O-α-L-rhamnopyranosyl-（1→2）-β-D-glucopyranosyl-dammar-20（24）-epoxy-3β,6α,12β,25-tetraol（Ⅰ）.6-O-（α-L-rhamnopyranosyl-（1→2）-β-D-glucopyranosyl）-20-O-β-D-glucopyranosyl-dammar-24-ene-3β,6α,12β,20S-tetraol （Ⅱ）.（14S）-3-（4,5-dihydroxy-6-（hydroxymethyl）-3-（3,4,5-trihydroxy-6-（hydroxy-methyl）-tetrahydro-2H-pyran-2-yloxy）-tetrahydro-2H-pyran-2-yloxy）-4,4,8,10,14-pentamethyl-17-（（S）-6-methyl-2-（3,4,5-trihydroxy-6-（（3,4,5-trihydroxy-6-（hydroxymethyl）-tetrahydro-2H-pyran-2-yloxy）methyl）-tetrahydro-2H-pyran-2-yloxy）hept-5-en-2-yl）-2,3,4,5,6,7,8,9,10,11,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-2-ylacetate（Ⅲ）.

关 键 词：西洋参 活性部位 胰岛素抵抗 化学成分 

分 类 号：R284.2[医药卫生—中药学]