非洲猪瘟病毒实时荧光定量PCR检测方法的建立  

Development of a real-time fluorescent quantitative PCR method for rapid detection of African swine fever virus

作　　者：黄萍[1] 肖家勇[1] 欧阳振宇[1] 陈盼[1] 白雪[1] 朱中武[1] 孟芳[1] 吴愈柏[2] 唐连飞[1] 

机构地区：[1]湖南出入境检验检疫局检验检疫技术中心,湖南长沙410004 [2]湖南出入境检验检疫局动物检疫处,湖南长沙410004

出　　处：《中国动物检疫》2010年第11期28-30,共3页China Animal Health Inspection

基　　金：湖南省科技厅资助(2008TP4012-2)

摘　　要：根据GenBank公布的非洲猪瘟病毒(ASFV)P72基因序列,设计了一对特异性引物和探针,使用含有选定检测序列的重组质粒标准品绘制标准曲线,建立了非洲猪瘟病毒实时荧光定量PCR检测方法。结果表明:该方法的敏感性可达100个拷贝值,具有良好的敏感性和重复性。A pair of specific primers and probe were designed according to the published sequence of P72 gene of African swine fever virus （ASFV）in GenBank and a series of dilutions of recombinant plasmids were prepared and used to generate standard curves, to establish a real-time fluorescent quantitative PCR method for detection of ASFV. The results showed that the PCR method was capable to detect 100 copies of African swine fever virus. The method was very sensitive and repeatable.

关 键 词：非洲猪瘟病毒 P72基因 实时荧光定量PCR 质粒 

分 类 号：S852.659.1[农业科学—基础兽医学]