荷叶黄酮类化合物提取工艺研究  

The Extraction Procedure of Total Flavonoids from Lotus Leaf

作　　者：邓国栋[1] 庄大海[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025

出　　处：《山地农业生物学报》2010年第5期428-431,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省科学技术基金资助项目[黔科合J字(2009)2321号];贵州大学博士基金资助项目[贵大人基合字(2009)008号]

摘　　要：以总黄酮得率为指标,利用单因素试验确定提取溶剂,通过正交试验法优化提取工艺参数。结果表明:以乙醇为溶剂,回流提取的最佳工艺条件为:提取温度70℃,乙醇体积分数45%,料液比1∶20,提取时间3 h,回流提取2次。在此条件下,总黄酮得率为8.8%,说明该提取工艺设计合理,稳定可行,操作简便,为荷叶黄酮的研究和开发提供了借鉴。To optimize the extraction procedure of total flavonoids from lotus leaf,the optimal extraction solvents and extraction process were examined by single factor test and orthogonal test,respectively.The optimal parameters were 45% ethanol,1∶20 solid-liquid ratio,2 times of extraction at 70℃ and each 3h,by which the total flavonoids yield rate was 8.8%.The extraction procedure was reliable and simple,which provided a basis for further exploitation of natural resource.

关 键 词：荷叶 总黄酮 提取工艺 优化 

分 类 号：R248.2[医药卫生—中医临床基础]