伴随中国番茄黄化曲叶病毒的卫星DNAβ在本氏烟中的种群变异  

Population Variation of Satellite DNAβ Associated with Tomato yellow leaf curl China virus in Nicotiana benthamiana

作　　者：任芳[1] 青玲[1] 熊艳[1] 孙现超[1] 

机构地区：[1]西南大学植物保护学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2010年第5期110-115,共6页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家青年科学基金资助项目(30700524)

摘　　要：采用根癌土壤杆菌介导接种和分子克隆技术,分析中国番茄黄化曲叶病毒(Tomato yellowleaf curl China virus,TYLCCNV)Y10分离物(Y10)的伴随卫星DNAβ(Y10β)在本氏烟中的种群变异.结果显示表明:本氏烟中Y10β种群所有克隆均发生不同程度的变异,在接种60 d及120 d后其种群突变频率分别为2.48×10-3和2.54×10-3,表明该卫星DNAβ在同一寄主体内的侵染时间对其种群变异水平没有明显影响;Y10β种群的碱基突变位点主要分布于DNAβ的富A(A-rich)区,在卫星共同区(SCR)内碱基突变位点最少;其碱基突变类型以A→G,G→A,T→C突变及碱基G缺失等突变类型的比例较高,并且在A-rich区内出现多个克隆在同一核苷酸位点发生相同突变的现象.The population variation of satellite DNAβ（Y10β） associated with Tomato yellow leaf curl China virus（TYLCCNV） isolate Y10 in Nicotiana benthamiana was analyzed,using the techniques of agroinoculation and molecular cloning.The results showed that mutations occurred in all clones of Y10β to different extent,and the mutation frequencies of Y10β populations at 60 and 120 days post-inoculation were 2.48×10-3 and 2.54×10-3 respectively,which indicated that the time course of infection for Y10β in the same host had no significant influence on its level of population variation.The mutations distributed mostly in the A-rich region of Y10β while the least number of mutations occurred in the satellite common region（SCR）.Among all the mutation types for Y10β populations in N.benthamiana,the base transitions from A to G,from G to A,from T to C and deletion of base G occupied higher proportions.Moreover,the phenomenon which several clones have the same mutation occurred at the same nucleotide position in A-rich region was observed in the Y10β populations.

关 键 词：中国番茄黄化曲叶病毒 卫星DNAΒ 种群变异 碱基突变 

分 类 号：S436.412.11[农业科学—农业昆虫与害虫防治]