温度和pH对条石鲷幼鱼消化酶活力的影响  

Effects of temperature and pH on the digestive enzymes activities of juvenile striped beakperch Oplegnathus fasciatus

作　　者：罗奇[1,2] 区又君[1] 艾丽[1,2] 李加儿[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,中国水产科学研究院水产种质资源与养殖技术重点开放实验室,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《热带海洋学报》2010年第5期154-158,共5页Journal of Tropical Oceanography

基　　金：中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放基金(2007A014)

摘　　要：研究了温度和pH对条石鲷Oplegnathus fasciatus幼鱼胃、肠、肝内3种主要消化酶(蛋白酶、淀粉酶和脂肪酶)活力的影响。结果表明,1)肠和肝的蛋白酶最适温度均为25℃,而胃蛋白酶最适温度为28℃;胃和肝淀粉酶最适温度是22℃,肠淀粉酶的最适温度为19℃;各消化器官的脂肪酶在16℃时活力最高。当温度在16-28℃之间时,条石鲷幼鱼的脂肪酶和蛋白酶活力较高,淀粉酶活力较低。2)胃蛋白酶最适pH为3.2,淀粉酶最适pH是7.6,脂肪酶最适pH为4.2;肠蛋白酶、淀粉酶和脂肪酶最适pH分别是8.6、6.6和6.6;肝蛋白酶最适pH是7.6,肝淀粉酶和脂肪酶最适pH均为6.6。The effects of temperature and pH on digestive enzymes activities（stomach,intestine and liver） were investigated in juvenile striped beakperch Oplegnathus fasciatus.The results indicated that the optimum temperature for protease in intestine and liver was 25℃,and that in stomach was 28℃.The optimum temperature for amylase in stomach and liver was 22℃,and that in intestine was 19℃.The optimum temperature for lipase in three digestive organs was all 16℃.During 16℃ to 28℃,the protease and lipase both had relatively high activities,however,the amylase had a lower activity.The optimum pH values for protease,amylase and lipase were 3.2,7.6 and 4.2 in stomach,and 8.6,6.6 and 6.6 in intestine,respectively.The optimum pH in liver was 7.6 for protease,and 6.6 for amylase and lipase.

关 键 词：条石鲷Oplegnathus fasciatus 幼鱼 温度 PH 消化酶 活力 

分 类 号：Q556[生物学—生物化学] Q48