用RP-HPLC法测定不同产地独一味药材中山栀子苷甲酯的含量  

Determination of content of shanzhiside methylester in Lamiophlomis rotata from different areas by RP-HPLC method

作　　者：樊鹏程[1] 李茂星[1] 贾正平[1] 张汝学[1] 邱建国[1] 

机构地区：[1]兰州军区兰州总医院药剂科,兰州730050

出　　处：《药学服务与研究》2010年第5期385-387,共3页Pharmaceutical Care and Research

基　　金：兰州军区医药卫生科研基金资助项目(No.LXH-200409);甘肃省科学技术攻关项目(No.2GS054-A43-014-14)

摘　　要：目的:建立测定不同产地独一味药材中山栀子苷甲酯含量的RP-HPLC方法。方法:采用SymmetryC18色谱柱(150mm×4.6mm,5μm),流动相:甲醇-水(25:75),流速:1.0ml/min,检测波长:238nm。结果:山栀子苷甲酯在5.0～50μg/ml(r=0.9998)线性关系良好,加样回收率为94.7%,RSD为2.8%。9批不同产地独一味药材中山栀子苷甲酯的含量为0.12%～1.30%,甘肃玛曲县所产独一味的除根以外的部分的山栀子苷甲酯的含量最高(1.30%)。结论:本方法准确、可靠,适于独一味药材中山栀子苷甲酯含量的测定。不同产地独一味药材中山栀子苷甲酯含量存在较大差异。Objective：To establish a RP-HPLC method for determination of shanzhiside methylester content in Lamiophlomis rotata from different areas.Methods：The separation was performed on a Symmetry C18 column（150 mm×4.6 mm,5 μm） with the mobile phase consisting of methanol-water（25：75）.The flow rate was 1.0 ml/min and the detection wavelength was 238 nm.Results：The standard curve was liner in the range of 5-50 μg/ml（r=0.999 8） for shanzhiside methylester.The recovery rate was 94.7% and RSD was 2.8%.The contents of shanzhiside methylester in 9 batches of Lamiophlomis rotata herb from different areas were from 0.12% to 1.30%.The content of Shanzhiside methylester in any parts except the root of Lamiophlomis rotata from Gansu Maqu was the highest of all（1.30%）.Conclusion：This method is accurate and reliable,and can be used for determination of shanzhiside methylester content in Lamiophlomis rotata.The content of shanzhiside methylester in Lamiophlomis rotata herb from different areas is various.

关 键 词：独一味 山栀子苷甲酯 含量测定 色谱法 高压液相 

分 类 号：R927.2[医药卫生—药学] R284