大鼠脑损伤后TNF-α及IL-10的研究及地塞米松对其影响  

The study of TNF-α and IL-10 in brain tissue of rat after brain injury and the effects of Dexamethasone

作　　者：张秀丽[1] 孙志扬[2] 

机构地区：[1]杭州师范大学附属第二人民医院,杭州310015 [2]上海市同济大学附属东方医院

出　　处：《浙江创伤外科》2010年第5期563-565,共3页Zhejiang Journal of Traumatic Surgery

摘　　要：目的观察大鼠脑损伤后TNF-α、IL-10的变化及注射地塞米松后对其影响。方法将28只SD大鼠按完全随机分组的方法分为:假手术组(4只);损伤组(12只);地塞米松干预组(12只);建立大鼠液压颅脑损伤模型,假手术组不予处理,损伤组给予液压打击处理,干预组予打击后立即腹腔注射地塞米松,采用ELISA法测定损伤区脑组织TNF-α、IL-10的表达。结果 TNF-α、IL-10的表达在损伤组较假手术组增高(P<0.05);但干预组较损伤组降低(P<0.05),且于损伤后6h、24h、48h期间表达增强(P<0.05)。结论 TNF-α、IL-10在大鼠损伤后表达增加,并在损伤6h、24h、48h中呈上升趋势,地塞米松可抑制其表达。Objective To observe the levels of TNF-α and IL-10 in brain tissue after injury of rat and the effects after injecting Dexamethasone.Methods according to compIete randomization princip1e,dividing twenty-eight S-D rats intosham operation group（four rats）、 trauma group（twelve rats） and Dexamethasone intervented group（twelve rats）;sustaining to mild TBI of rat,sham operation group is treated with nothing;trauma group is treated with hydraulic combat;Dexamethasone intervented group is injected Dexamethasone into intraperitoneal immediately after trauma,to detect the levels of TNF-α and IL-10 in brain tissue extracts by ELISA.Results the levels of TNF-α and IL-10 are higher in trauma group than that in sham operation group and also higher in trauma group than that in in Dexamethasone intervented group（P〈0.05）and they are rising after trauma during 6h to 48h（P〈0.05）.Conclusion the levels of TNF-α and IL-10 are rising after trauma from 6h to 48h,Dexamethasone can inhibit their levels.

关 键 词：脑损伤 TNF-Α IL-10 地塞米松 

分 类 号：R512.62[医药卫生—内科学]