拟南芥高迁移率族蛋白B族基因At2G34450的表达及功能鉴定  

The Heterologous Expression and Functional Identification of the Gene At2G34450 in High Mobility Group B Proteins of Arabidopsis thaliana

作　　者：冀芦沙[1] 贺立龙[1] 王洪霞[1] 郭尚敬[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《安徽农业科学》2010年第27期15071-15073,15075,共4页Journal of Anhui Agricultural Sciences

基　　金：聊城大学博士启动经费(31805)

摘　　要：[目的]研究高等真核生物细胞核中的高迁移率族蛋白B(HMGB)在植物胁迫反应的转录调控中的作用方式。[方法]克隆了拟南芥中编码HMGB蛋白的基因At2G33450,将其转化拟南芥并筛选出超表达的转基因植株,检测干旱、高盐等非生物胁迫对转基因拟南芥的影响。[结果]在盐或干旱胁迫下,过度表达At2G33450的转基因拟南芥与野生型相比,出现萌发及生长迟缓现象。[结论]HMGB蛋白家族中的At2G33450蛋白对各种胁迫条件下拟南芥的生长发育具有重要作用。[ Objective ] The aim was to study the action mode of high mobility group B （HMGB） proteins of higher eukaryotic cells in the transcriptional regulation of plant stress responses. [ Method ] The At2G33450 gene encoding AT2G34450 protein in Arabidopsis thaliana was eloned. Then, binary vectors carrying the gene were transformed into Arabidopsis thaliana and the over-expression strains were selected to detect the influences of environmental stimuli on transgenic Arabidopsis. [ Result ] Under salt or drought stress, the transgenic Arabidopsis plants which over-expressed At2G33450 displayed retarded germination and subsequent growth compared with wild-type plants. [ Conclusion ] At2G33450 protein of HMGB protein family played an important role in the growth and development of Arabidopsis plants under various stress conditions.

关 键 词：非生物胁迫 拟南芥 转基因植物 高迁移率族蛋白 

分 类 号：S41[农业科学—植物保护]