水果型番木瓜组织培养的研究  

Tissue Culture of Mini-Papaya

作　　者：谢恩倍[1] 欧善生[1] 姚军[1] 石梅娟[1] 梁盛[1] 朱国兵[1] 闵华[1] 赵广存[1] 

机构地区：[1]广西农业职业技术学院,广西南宁530007

出　　处：《安徽农业科学》2010年第28期15495-15497,共3页Journal of Anhui Agricultural Sciences

基　　金：广西农业职业技术学院科学研究与技术开发计划项目(农职科B070210)

摘　　要：[目的]研究水果型番木瓜的组织培养。[方法]以经预处理的种子为试材,通过MS基本培养基附加不同浓度的6-BA和IBA,对水果型番木瓜组培快繁技术进行了研究。[结果]无菌条件下用75%酒精均匀擦拭水果型番木瓜果皮表面,取出种子,用无菌水冲洗3次是较好的灭菌方法,种子污染率仅为2.52%;水果型番木瓜种子经1000mg/LGA3+1mg/L6-BA(等体积混合)浸泡18h后接种于MS培养基中培养,种子发芽率、胚芽长度、胚根长度和苗高达到较高值,分别为68.42%、2.25cm、0.80cm和1.52cm,幼苗整体生长较好;继代增殖培养基为MS+0.5mg/L6-BA+0.1mg/LIBA时增殖系数最高(7.92),幼苗长势较好;组培苗玻璃化随着光照强度的增强而呈减弱趋势,3000lx处理下玻璃化苗比率最低(3.21%)。[结论]该研究为水果型番木瓜的组培快繁研究提供了参考。[Objective]The aim was to study the tissue culture of mini-Papaya.[Method]The pretreatment seeds of mini-Papaya were cul-tured in the MS medium containing 6-BA and IBA of different densities for rapid propagation.[Result]In the condition of aseptic strain,the surface of mini-Papaya peel was uniformly wiped by 75% alcohol,then seeds were removed and washed by aseptic water for 3 times,which was the best sterilization method,and the pollution rate of seed was only 2.52% .After seeds which had been soaked by the equivolume mix-solution of 1 000 mg/L GA3 and 1 mg/L 6-BA for 18 h were further purified in MS medium,the germination rate of seed,the length of germ and radical and the height of seedling were 68.42% ,2.25 cm,0.80 cm and 1.52 cm,respectively,furthermore the total situation of seedling growth was better.When subculture multiplication medium was MS ＋ 0.5mg/L 6-BA ＋ 0.1 mg/L IBA medium,proliferation coefficient of sub-culture multiplication reached the highest （7.92） ,and the seedings grew better.The ratio of vitrified shoots decreasing with the increase of light intensity could reach the lowest level （3.21% ） under the light intensity of 3 000 lx.[Conclusion]The research provides reference for studies on tissue culture and rapid propagation of mini-Papaya.

关 键 词：番木瓜 种子 GA3 6-BA 组培 

分 类 号：S661.6[农业科学—果树学]