10个黄色金针菇菌株的栽培特性及亲缘关系分析  

Cultivation Characteristics and Kinship Analysis on 10 Strains of Yellow Flammulina velutiper (Fr.) Sing

作　　者：崔鹏[1] 通占元 张树斌[1] 王朝江[3] 王立安[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]河北农业环境保护监测站,河北石家庄050011 [3]河北农林科学院遗传生理所,河北石家庄050051

出　　处：《安徽农业科学》2010年第28期15510-15512,15514,共4页Journal of Anhui Agricultural Sciences

基　　金：河北省科技支撑计划项目(No.08220201D)

摘　　要：采用棉籽壳熟料袋栽模式对10个黄色金针菇菌株进行品比试验,采用酯酶同工酶图谱和RAPD技术分析菌株间的遗传特性及亲缘关系。结果表明,6号(黄064)菌株的菌丝生长速度、满袋时间、第1潮菇生物学效率、子实体成熟时间等指标表现优良,适合作为工厂化栽培优良菌种;10号(Bj-3)菌株子实体形态特征较好,适合作为优良品种推广。酯酶同工酶图谱和RAPD技术分析结果基本一致,10个菌株中6(黄064)、7号(三明B)菌株为同种异名菌株。The fermented cottonseed hull-in-bag pattern was used in the variety comparative test on 10 strains of yellow Flammulina velutiper （Fr.） Sing and the hereditary characters and kinship among the strains were analyzed by applying esterase isozyme electrophorogram and RAPD technique.The results showed that the strain No.6 （Huang064） showed good values of indexes,such as mycelial growth speed,sackfull time,biological efficiency of first harvested mushroom and mature time of fruiting body,so it was suitable for industrial cultivation as good strain; the morphological characters of fruiting body of the strain No.10 （Bj-3） were better and it was suitable for popularization as good variety.The esterase isozyme electrophorogram was basically consistent with results of RAPD technical analysis.Among the 10 strains,the strain No.6 （Huang064） and No.7 （SanmingB） were allogenic synonym strains

关 键 词：黄色金针菇 品比试验 酯酶同工酶 RAPD 聚类分析 

分 类 号：S646.15[农业科学—蔬菜学]