湖北小连翘与贯叶连翘中金丝桃素的含量比较  

Comparison of Hypericin Contents in Hypericum hubeiense and Hypericum perforatum

作　　者：李云和[1] 王发松[2] 

机构地区：[1]中山大学肿瘤防治中心,广东广州510060 [2]湖北民族学院化学与环境工程学院,湖北恩施445000

出　　处：《安徽农业科学》2010年第28期15598-15599,共2页Journal of Anhui Agricultural Sciences

基　　金：湖北省教育厅科技研究计划优秀中青年人才项目(Q20082901)

摘　　要：[目的]比较测定贯叶连翘和湖北小连翘中金丝桃素的含量,以期为湖北小连翘这一新金丝桃素植物资源的开发利用奠定基础。[方法]采用HPLC法测定金丝桃素含量,色谱条件为:Supelcosi lODS色谱柱(4.6mm×250mm,5μm),流动相为甲醇-乙腈-水-0.3%磷酸,(V∶V=45∶50∶4.5∶0.5,用三乙胺调pH值至6.0),流速为1ml/min,检测波长为590nm。[结果]相同生长环境下的湖北小连翘中金丝桃素含量约为贯叶连翘中的3倍;湖北小连翘中金丝桃素的含量随着海拔高度的升高呈逐渐下降趋势;金丝桃素在湖北小连翘各部位中以花和幼果中含量最高,可达0.617%～0.877%,在叶和成熟果实中的含量约为0.35%,根和茎中含量极低。[结论]该试验为湖北小连翘的人工种植和科学采收研究提供了理论指导,为湖北小连翘这一新金丝桃素植物资源的开发利用奠定基础。[Objective]The contents of hypericin in Hypericum hubeiense and Hypericum perforatum were comparatively determined,aiming at providing basis for the development and utilization of Hypericum hubeiense.[Method]HPLC was used,and the chromatographic conditions were as follows：Supelcosil ODS （4.6 mm×250 mm,5 μm） column,mobile phase of methanol-acetonitrile-water-0.3% aqueous solution of phosphoric acid （v∶v=45∶50∶4.5∶0.5,adjusting the pH to 6 with triethylamine） ,flow rate of 1 ml/min and detection wavelength of 590 nm.[Result]The content of hypericin in Hypericum hubeiense was 3 times as that in Hypericum perforatum growing in the same environ-ment and decreased with the increasing elevation of its growth.Meanwhile,the hypericin content in the flowers and young fruits of Hypericum hubeiense was the highest which was 0.617%-0.877% ,and that in leaves and ripe fruits was about 0.35% ,while that in roots and stems was extremely low.[Conclusion]This will provide theoretical basis for the artificial cultivation,scientific harvest and development of Hypericum hubeiense.

关 键 词：湖北小连翘 贯叶连翘 金丝桃素 含量比较 

分 类 号：S682.36[农业科学—观赏园艺]