甘肃省不同区域当归种子的RAPD分析  

RAPD Analysis of Six Different Regions Angelicae sinensis Seed in Gansu

作　　者：周鹤峰[1] 邵敏[1] 姬可平[1] 李啸红[1] 李应东 晋玲 

机构地区：[1]遵义医学院珠海校区,广东珠海519041 [2]甘肃省中医学院,甘肃兰州730000

出　　处：《安徽农业科学》2010年第28期15616-15617,共2页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BAI37B01)

摘　　要：[目的]探讨RAPD技术应用于甘肃省当归[Angelicae sinensis(Oliv.)Diels)品种鉴别的可行性。[方法]采用改良CTAB法提取甘肃省不同地区的当归种子基因组DNA,利用筛选的引物S61分别对其进行RAPD分析。[结果]获得了质量较高的当归种子基因组DNA,引物S61扩增的图谱显示出良好的多态性,且比较稳定,重复性好。[结论]该法可为从分子生物学角度鉴别当归提供科学依据。[Objective]To explore the feasibility of using RAPD analysis to identify Angelicae sinensis in Gansu.[Methods] The genomic DNAs were extracted from Angelicae sinensis by modified CTAB method and analyzed by RAPD with primer S61.[Result]It had obtained high quality team genomic DNA by modified CTAB method.The RAPD map showed that high polymorphism existed in primer S61 was clear and repetitive.[Conclusion]The results obtained provided the basis for identifing Angelicae sinensis from the level of molecular biology.

关 键 词：当归种子 DNA提取 改良CTAB法 RAPD 

分 类 号：S567.239[农业科学—中草药栽培]