益智液相指纹图谱的研究  

Study on Fingerprints of Alpinia oxyphylla Miq. by HPLC

作　　者：刘红[1] 周婧然[1] 毕和平[1] 韩长日[1] 

机构地区：[1]海南师范大学化学与化工学院,海南海口571158

出　　处：《安徽农业科学》2010年第28期15623-15623,15626,共2页Journal of Anhui Agricultural Sciences

基　　金：海南省教育厅高等学校自然科学资助项目(hjkj200619);海南师范大学应用化学特色专业建设项目(HSZJ0608)

摘　　要：[目的]采用高效液相色谱法建立益智的指纹图谱。[方法]采用HPLC检测,检测条件为:分析柱为岛津VP-ODS(150L×4.6mm),流动相为水-乙腈(v∶v=7∶30),进样量为20μl,检测波长为220nm。[结果]海南的益智指纹谱图相似度较大,与广西和广东的益智样品有一定的差异;广东生长的益智和大部分地区的益智有着很大的相似性;而广西的益智在总体上也和各地区的益智相似。[结论]该试验建立了10种不同产地益智的液相指纹图谱,该方法方便、快捷、准确、可靠,可以直接指导实际生产,会成为益智药材快速鉴别的一种新方法。[Objective]The aim was to establish the fingerprints of Alpinia oxyphylla Miq.using HPLC.[Method]The HPLC was used and the chromatographic conditions were as follows：column of ODS Hypersil VP-ODS （150 L×4.6 mm） ,mobile phases of water-acetonitrile （v∶v=70∶30） ,injecting of 20 ul and detection wavelength of 220 nm.[Result]The fingerprints of Alpinia oxyphylla Miq.obtained from Hainan province were similar and different from that obtained from Guangdong province and Guangxi province.The fingerprints of Alpinia oxyphylla Miq.obtained from Guangdong province and Guangxi province were similar as that obtained from the other areas.[Conclusion]The 10 kinds of fingerprints were obtained by this rapid,convenient and reliable method,which would be a new rapid identifying method for Alpinia oxyphylla Miq..

关 键 词：益智 指纹谱图 HPLC 

分 类 号：S567.9[农业科学—中草药栽培]