赤霉素诱导甘蔗节间伸长基因的cDNA-SCoT差异表达分析  

Differential Expression of Gibberellin-Induced Genes for Stalk Elongation of Sugarcane Analyzed with cDNA-ScoT

作　　者：吴建明[1,2,3,4] 李杨瑞[1,2,3,4] 王爱勤[3,4] 杨柳[1,2,3,4] 杨丽涛[3,4] 

机构地区：[1]广西甘蔗研究所,广西南宁530007 [2]广西作物遗传改良生物技术重点开发实验室,广西南宁530007 [3]广西大学广西亚热带生物资源保护利用重点实验室,广西南宁530004 [4]中国农业科学院甘蔗研究中心,广西南宁530007

出　　处：《作物学报》2010年第11期1883-1890,共8页Acta Agronomica Sinica

基　　金：国家科技支撑计划项目(2007BAD30B03);广西科技攻关重点项目(桂科攻0782004-3);广西科技攻关项目(桂科能0630006-5F;桂科能0815011);现代农业产业技术体系建设专项资金(nycytx-024-01-15)资助

摘　　要：以甘蔗ROC22为材料,在伸长初期进行叶面喷施200mgL?1浓度的赤霉素为处理,以喷施清水为对照,在不同时间取幼茎样品,用cDNA-SCoT进行基因差异表达分析。结果表明,通过46条引物筛选了约700个cDNA片段,获得差异片段30个。有18个基因片段受赤霉素上调而12个基因片段下调;其中16个TDFs序列和NCBI数据库中已录入的基因具有较高的相似性,按照其功能可分为6类,即能量与代谢相关基因(占总数的20.0%)、未知功能蛋白(占总数的20.0%)、未知基因(占总数的46.7%)、信号传导相关基因(占总数的6.7%)、转录因子相关基因(占总数的3.3%)和细胞凋亡基因(3.3%)。这说明利用SCoT方法可以进行蔗茎基因差异表达研究,分离到的一些差异片段可能是与甘蔗节间伸长相关的基因。The present study was conducted to investigate the molecular mechanisms of gibberellin-induced stalk internodes elongation in sugarcane.Using sugarcane variety ROC22,the plant leaves were treated with spraying gibberellic acid（GA3） at 200 mg L-1 at early elongation stage,and spraying water as a control.Samples of young stalks were taken and the differentially expressed genes were analyzed with cDNA-SCoT technique.The results showed that 30 fragments of differentially expressed genes were obtained by screening 700 cDNA fragments with 46 single primers.The expressions of 18 genes were up-regulated,and 12 genes down-regulated by GA3 induction,and the sequences of 16 TDFs showed high similarity to the registered genes in the NCBI database.These genes were divided into six categories by functional analysis,including energy and metabolism-related genes（accounting for 20.0%）,unknown functional proteins（accounting for 20.0%）,unknown genes（accounting for 46.7%）,signal transduction-related genes（accounting for 6.7%）,transcription factor-related genes（accounting for 3.3%）,and cell apoptosis（accounting for 3.3%）.This suggested that the differential expression of genes in the stalks can be analyzed with SCoT technique,and some fragments may be related to the stalk elongation of surgarcane.

关 键 词：赤霉素 甘蔗 cDNA-SCoT 差异表达 

分 类 号：S566.1[农业科学—作物学]